Cardinal temperatures and thermal time for seed germination of Brunonia Australis (Goodeniaceae) and calandrinia sp. (Portulacaceae)

Seed germination of Brunonia australis Sm. ex R.Br. and Calandrinia sp. (Mt. Clere: not yet fully classified) was investigated using a thermogradient plate set at different constant temperatures to determine seed propagation requirements of these potential floriculture species. Germination responses were tested at 3, 7, 11, 15, 18, 22, 25, 29, 34, and 38 °C. Germination data were modeled using the cumulative distribution function of the inverse normal, which provides information on lag, rate, and maximum seed germination for each temperature regime. To determine cardinal temperatures, the reciprocal time to median germination (1/t₅₀) and percentage germination per day were calculated and regressed against temperature. Base temperature estimates for B. australis were 4.9 and 5.5 °C and optimum temperatures were 21.4 and 21.9 °C, whereas maximum temperatures were 35.9 and 103.5 °C, with the latter being clearly overestimated using the 1/t₅₀ index. Base temperatures for Calandrinia sp. were 5.8 and 7.9 °C, whereas optimum and maximum temperature estimates of 22.5 and 42.7 °C, respectively, were reported using the percentage germination per day index. Maximum seed germination of 0.8 to 0.9, expressed as the probability of a seed germinating, occurred at 11 to 25 °C for B. australis, whereas maximum germination for Calandrinia sp. was 0.5 to 0.7 at 18 to 25 °C. Thermal time, the accumulation of daily mean temperate above a base temperature, was calculated for different germination percentages. Estimates of thermal time (°Cd) for 50% seed germination were 54 and 90 °Cd for B. australis and Calandrinia sp., respectively.