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Biochemical properties of a keratan sulphate/chondroitin sulphate proteoglycan expressed in primate pluripotent stem cells

Citation

Cooper, S and Bennett, W and Andrade, J and Reubinoff, BE and Thomson, J and Pera, MF, Biochemical properties of a keratan sulphate/chondroitin sulphate proteoglycan expressed in primate pluripotent stem cells , Journal of Anatomy: Molecular, Cellular and Experimental Morphology, 200, (3) pp. 259-265. ISSN 0021-8782 (2002) [Refereed Article]

DOI: doi:10.1046/j.1469-7580.2002.00034.x

Abstract

We previously identified a pericellular matrix keratan sulphate/chondroitin sulphate proteoglycan present on the surface of human embryonal carcinoma stem cells, cells whose differentiation mimics early development. Antibodies reactive with various epitopes on this molecule define a cluster of differentiation markers for primate pluripotent stem cells. We describe the purification of a form of this molecule which is secreted or shed into the culture medium. Biochemical analysis of the secreted form of this molecule shows that the monomeric form, whilst containing keratan sulphate, resembles mucins in its structure and its modification with O-linked carbohydrate. Immunofluorescence and immunoblotting data show that monkey and human pluripotent stem cells react with antibodies directed against epitopes on either carbohydrate side chains or the protein core of the molecule.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Cell Development, Proliferation and Death
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Bennett, W (Dr Bill Bennett)
ID Code:63805
Year Published:2002
Web of Science® Times Cited:19
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-06-01
Last Modified:2011-08-02
Downloads:0

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