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Structural-proliferative units and organ growth: effects of insulin-like growth factor 2 on the growth of colon and skin

Citation

Bennett, WR and Crew, TE and Slack, JMW and Ward, A, Structural-proliferative units and organ growth: effects of insulin-like growth factor 2 on the growth of colon and skin , Development (Cambridge), 130, (6) pp. 1079-1088. ISSN 0950-1991 (2003) [Refereed Article]

DOI: doi:10.1242/dev.00333

Abstract

Many epithelial renewal tissues in vertebrates are organised into structural-proliferative units. We have examined the effect of IGF2 dose on the structure of structural-proliferative units in skin and colon. The mouse strains used were the Igf2 knockout, wild type and K:Igf2, a transgenic in which Igf2 is overexpressed under control of a keratin promoter. For both skin and colon, the histological organisation of structural-proliferative units was unaltered with increasing IGF2 dose, although there was a higher fraction of dividing cells in the proliferative compartment. In the colon an increase in IGF2 dose increases the overall area of the epithelium. This is due to an increase in the number of crypts with no change of cell size or of crypt area. Growth stimulation appears to be due to a reduction in the duration of crypt fission. The conclusion is that the IGF2 pathway can stimulate the multiplication of colonic crypts independently of stimulating increased cell proliferation. The results for the skin are consistent with this. An increase of IGF2 dose increases the proportion of dividing cells in the basal layer, the thickness of the epidermis and the total area of the epidermis. By comparison with Drosophila, these results show no effects on cell size, but do show the possibility of inducing disproportionate growth. These differences may represent properties of the SPU organisation that is characteristic of vertebrate tissues.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Developmental Genetics (incl. Sex Determination)
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Skin and Related Disorders
Author:Bennett, WR (Dr Bill Bennett)
ID Code:63803
Year Published:2003
Web of Science® Times Cited:31
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-06-01
Last Modified:2011-08-01
Downloads:0

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