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Kinetic performance appraisal of poly(styrene-co-divinylbenzene) monolithic high-performance liquid chromatography columns for biomolecule analysis

Citation

Causon, TJ and Shellie, RA and Hilder, EF, Kinetic performance appraisal of poly(styrene-co-divinylbenzene) monolithic high-performance liquid chromatography columns for biomolecule analysis, Journal of Chromatography A, 1217, (24) pp. 3765-3769. ISSN 0021-9673 (2010) [Refereed Article]


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DOI: doi:10.1016/j.chroma.2010.04.007

Abstract

A broad appraisal of the kinetic performance of organic polymeric monolithic columns is reported using commercially available poly(styrene-co-divinylbenzene) monolithic columns (Dionex ProSwiftĘ‚ RP-1S). Analysis of a protein digest sample at elevated temperatures (°√80 °∆C) indicated no apparent analyte degradation using an inert polymeric stationary phase. Comparison between low molecular weight solute and peptide separations highlighted the markedly different mass transport processes observed on macroporous monolithic beds and an improved C term at elevated temperature in both instances. The current usefulness of this column format for biomolecule analysis was further studied via employment of a kinetic performance characterisation for the first time to provide direction for column development servicing this application.

Item Details

Item Type:Refereed Article
Keywords:Polymer monoliths; Kinetic performance; Bioseparations; Peptides; High temperature liquid chromatography
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Causon, TJ (Mr Timothy Causon)
Author:Shellie, RA (Associate Professor Robert Shellie)
Author:Hilder, EF (Professor Emily Hilder)
ID Code:63736
Year Published:2010
Funding Support:Australian Research Council (DP0987318)
Web of Science® Times Cited:15
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-05-26
Last Modified:2015-01-20
Downloads:0

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