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Electroosmotic flow-balanced isotachophoretic stacking with continuous electrokinetic injection for the concentration of anions in high conductivity samples

Citation

Breadmore, MC, Electroosmotic flow-balanced isotachophoretic stacking with continuous electrokinetic injection for the concentration of anions in high conductivity samples , Journal of Chromatography A, 1217, (24) pp. 3900-3906. ISSN 0021-9673 (2010) [Refereed Article]


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DOI: doi:10.1016/j.chroma.2010.04.011

Abstract

An EOF counter-balanced ITP boundary has been used to stack anions from high conductivity samples during continuous electrokinetic injection of the sample. In a polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, the time at which the ITP boundary exited the capillary could be prolonged by balancing the movement of the boundary with the EOF. Using a bis-tris-propane electrolyte, the ITP boundary was removed from the capillary within 7 min, while when using triethanolamine the ITP boundary was still at 30% of the capillary after 2 h of injection. Using these systems, the sensitivity of a mixture of simple organic acids in 100 mM Cl− was improved by 700800-fold using bis-tris-propane with a whole-capillary injection of the sample and 5 min of electrokinetic injection at +28 kV, and 11001300-fold using triethanolamine and 60 min of electrokinetic injection under the same conditions. The potential of the method to be applicable to high conductivity samples was demonstrated by stacking a whole capillary filled with urine spiked with naphthalenedisulfonic acid, with limits of detection 450 times lower than those achievable with a normal hydrodynamic injection.

Item Details

Item Type:Refereed Article
Keywords:Capillary electrophoresis; Stacking; High salt stacking; Urine; Biological fluids
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:63734
Year Published:2010
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:20
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-05-26
Last Modified:2011-05-04
Downloads:0

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