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Pseudomonas aeruginosa quorum-sensing signal molecules interfere with dendritic cell-induced T-cell proliferation

Citation

Skindersoe, ME and Zeuthen, LH and Brix, S and Fink, LN and Lazenby, J and Whittall, C and Williams, P and Diggle, SP and Froekiaer, H and Cooley, M and Givskov, M, Pseudomonas aeruginosa quorum-sensing signal molecules interfere with dendritic cell-induced T-cell proliferation, F E M S Immunology and Medical Microbiology: (Federation of European Microbiological Societies), 55, (3) pp. 335-345. ISSN 0928-8244 (2009) [Refereed Article]

DOI: doi:10.1111/j.1574-695X.2008.00533.x

Abstract

Pseudomonas aeruginosa releases a wide array of toxins and tissue-degrading enzymes. Production of these malicious virulence factors is controlled by interbacterial communication in a process known as quorum sensing. An increasing body of evidence reveals that the bacterial signal molecule N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) exhibits both quorum-sensing signalling and immune-modulating properties. Recently, yet another quorum-sensing signal molecule, the Pseudomonas quinolone signal (PQS), has been shown to affect cytokine release by mitogen-stimulated human T cells. In the present article we demonstrate that both OdDHL and PQS decrease the production of interleukin-12 (IL-12) by Escherichia coli lipopolysaccharide-stimulated bone marrow-derived dendritic cells (BM-DCs) without altering their IL-10 release. Moreover, BM-DCs exposed to PQS and OdDHL during antigen stimulation exhibit a decreased ability to induce T-cell proliferation in vitro. Collectively, this suggests that OdDHL and PQS change the maturation pattern of stimulated DCs away from a proinflammatory T-helper type I directing response, thereby decreasing the antibacterial activity of the adaptive immune defence. OdDHL and PQS thus seem to possess dual activities in the infection process: as inducers of virulence factors as well as immune-modulators facilitating the infective properties of this pathogen. © 2009 Federation of European Microbiological Societies.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Infectious Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Cooley, M (Associate Professor Margaret Cooley)
ID Code:63729
Year Published:2009
Web of Science® Times Cited:32
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-05-26
Last Modified:2010-05-26
Downloads:0

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