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Perivascular cells in a skin graft are rapidly repopulated by host cells

Citation

O'Ceallaigh, S and Herrick, SE and Bennett, WR and Bluff, JE and Ferguson, MW and McGrouther, DA, Perivascular cells in a skin graft are rapidly repopulated by host cells, Journal of Plastic, Reconstructive & Aesthetic Surgery , 60, (8) pp. 864-875. ISSN 1748-6815 (2007) [Refereed Article]

DOI: doi:10.1016/j.bjps.2006.03.036

Abstract

Survival of grafted tissues is dependent upon revascularisation. This study investigated revascularisation in a murine skin graft model, using two methods. The first involved 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine (DiI) labelling of the wound bed, prior to replacing the skin graft, to allow tracking of host cells into the grafts. At time points between day 3 and day 14 post-surgery, DiI-labelled cells which had tracked into the grafts, were found to co-localise with CD31 positive endothelial cells and patent perfused vessels (fluorescein isothiocyanate (FITC)-dextran perfusion), to show possible association with the vasculature. To further differentiate between graft and host-derived cells, C57BL/6 wild-type grafts were placed on enhanced-green fluorescent protein (e-GFP) transgenic mouse hosts, and at set times post-grafting examined using confocal microscopy. Patent vessels were found at all depths of the graft by day 3. Host (DiI- or GFP-positive) cells were predominantly co-localised with graft vessels in grafts from day 3 onwards, with a similar morphology to control skin. Significantly more GFP labelled host cells were visualised in the superficial dermis at day 5 compared to day 3. Initial restoration of circulation appears to be due to linkage between existing graft and bed vessels, followed by an influx of host cells with a definite perivascular distribution. These findings have implications for skin autografts and tissue engineered skin substitutes. © 2006 British Association of Plastic, Reconstructive and Aesthetic Surgeons.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Cell Development, Proliferation and Death
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Skin and Related Disorders
Author:Bennett, WR (Dr Bill Bennett)
ID Code:63717
Year Published:2007
Web of Science® Times Cited:7
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-05-26
Last Modified:2011-07-29
Downloads:0

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