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Perivascular cells in a skin graft are rapidly repopulated by host cells
Citation
O'Ceallaigh, S and Herrick, SE and Bennett, WR and Bluff, JE and Ferguson, MW and McGrouther, DA, Perivascular cells in a skin graft are rapidly repopulated by host cells, Journal of Plastic, Reconstructive & Aesthetic Surgery , 60, (8) pp. 864-875. ISSN 1748-6815 (2007) [Refereed Article]
DOI: doi:10.1016/j.bjps.2006.03.036
Abstract
Survival of grafted tissues is dependent upon revascularisation. This study investigated revascularisation in a murine skin graft model, using two methods. The first involved 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine (DiI) labelling of the wound bed, prior to replacing the skin graft, to allow tracking of host cells into the grafts. At time points between day 3 and day 14 post-surgery, DiI-labelled cells which had tracked into the grafts, were found to co-localise with CD31 positive endothelial cells and patent perfused vessels (fluorescein isothiocyanate (FITC)-dextran perfusion), to show possible association with the vasculature. To further differentiate between graft and host-derived cells, C57BL/6 wild-type grafts were placed on enhanced-green fluorescent protein (e-GFP) transgenic mouse hosts, and at set times post-grafting examined using confocal microscopy. Patent vessels were found at all depths of the graft by day 3. Host (DiI- or GFP-positive) cells were predominantly co-localised with graft vessels in grafts from day 3 onwards, with a similar morphology to control skin. Significantly more GFP labelled host cells were visualised in the superficial dermis at day 5 compared to day 3. Initial restoration of circulation appears to be due to linkage between existing graft and bed vessels, followed by an influx of host cells with a definite perivascular distribution. These findings have implications for skin autografts and tissue engineered skin substitutes. © 2006 British Association of Plastic, Reconstructive and Aesthetic Surgeons.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and cell biology |
| Research Field: | Cell development, proliferation and death |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | Bennett, WR (Dr Bill Bennett) |
| ID Code: | 63717 |
| Year Published: | 2007 |
| Web of Science® Times Cited: | 10 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2010-05-26 |
| Last Modified: | 2011-07-29 |
| Downloads: | 0 |
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