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Olfactory ensheathing cell-produced SPARC modulates Schwann cell activation to promote neurite outgrowth and spinal cord repair

Citation

Au, E and Richter, MW and Vincent, AJ and Tetzlaff, W and Aebersold, R and Sage, EH and Roskams, AJ, Olfactory ensheathing cell-produced SPARC modulates Schwann cell activation to promote neurite outgrowth and spinal cord repair, Journal of Neuroscience, 27, (27) pp. 7208-7221. ISSN 0270-6474 (2007) [Refereed Article]

DOI: doi:10.1523/JNEUROSCI.0509-07.2007

Abstract

Olfactory ensheathing cells (OECs) transplanted into the lesioned CNS can stimulate reportedly different degrees of regeneration, remyelination, and functional recovery, but little is known about the mechanisms OECs may use to stimulate endogenous repair. Here, we used a functional proteomic approach, isotope-coded affinity tagging and mass spectrometry, to identify active components of the OEC secreteome that differentially stimulate outgrowth. SPARC (secreted protein acidic rich in cysteine) (osteonectin) was identified as an OEC-derived matricellular protein that can indirectly enhance the ability of Schwann cells to stimulate dorsal root ganglion outgrowth in vitro. SPARC stimulates Schwann cell-mediated outgrowth by cooperative signal with laminin-1 and transforming growth factor β. Furthermore, when SPARC-null OECs were transplanted into lesioned rat spinal cord, the absence of OEC-secreted SPARC results in an attenuation of outgrowth of specific subsets of sensory and supraspinal axons and changes the pattern of macrophage infiltration in response to the transplanted cells. These data provide the first evidence for a role for SPARC in modulating different aspects of CNS repair and indicate that SPARC can change the activation state of endogenous Schwann cells, resulting in the promotion of outgrowth in vitro, and in vivo. Copyright © 2007 Society for Neuroscience.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Vincent, AJ (Dr Adele Vincent)
ID Code:63663
Year Published:2007
Web of Science® Times Cited:83
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-05-20
Last Modified:2010-05-20
Downloads:0

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