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High throughput analysis of red wine and grape Phenolics - Adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format
Citation
Mercurio, MD and Dambergs, RG and Herderich, MJ and Smith, PA, High throughput analysis of red wine and grape Phenolics - Adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format, Journal of Agricultural and Food Chemistry, 55, (12) pp. 4651-4657. ISSN 0021-8561 (2007) [Refereed Article]
Abstract
The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Agricultural and Veterinary Sciences |
| Research Group: | Horticultural Production |
| Research Field: | Oenology and Viticulture |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Industrial Crops |
| Objective Field: | Wine Grapes |
| Author: | Dambergs, RG (Dr Robert Dambergs) |
| ID Code: | 63459 |
| Year Published: | 2007 |
| Web of Science® Times Cited: | 95 |
| Deposited By: | Agricultural Science |
| Deposited On: | 2010-05-07 |
| Last Modified: | 2010-05-10 |
| Downloads: | 0 |
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