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High throughput analysis of red wine and grape Phenolics - Adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format
journal contribution
posted on 2023-05-17, 02:31 authored by Mercurio, MD, Robert Dambergs, Herderich, MJ, Smith, PAThe methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.
History
Publication title
Journal of Agricultural and Food ChemistryVolume
55Issue
12Pagination
4651-4657ISSN
0021-8561Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Amer Chemical SocPlace of publication
1155 16Th St, Nw, Washington, USA, Dc, 20036Repository Status
- Restricted