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High throughput analysis of red wine and grape Phenolics - Adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format

Citation

Mercurio, MD and Dambergs, RG and Herderich, MJ and Smith, PA, High throughput analysis of red wine and grape Phenolics - Adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format, Journal of Agricultural and Food Chemistry, 55, (12) pp. 4651-4657. ISSN 0021-8561 (2007) [Refereed Article]

DOI: doi:10.1021/jf063674n

Abstract

The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Horticultural Production
Research Field:Oenology and Viticulture
Objective Division:Plant Production and Plant Primary Products
Objective Group:Industrial Crops
Objective Field:Wine Grapes
Author:Dambergs, RG (Dr Robert Dambergs)
ID Code:63459
Year Published:2007
Web of Science® Times Cited:101
Deposited By:Agricultural Science
Deposited On:2010-05-07
Last Modified:2010-05-10
Downloads:0

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