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Variability in biofilm production by Listeria monocytogenes correlated to strain origin and growth conditions

Citation

Nilsson, RE and Ross, T and Bowman, JP, Variability in biofilm production by Listeria monocytogenes correlated to strain origin and growth conditions, International Journal of Food Microbiology, 150, (1) pp. 14-24. ISSN 0168-1605 (2011) [Refereed Article]

Copyright Statement

Copyright © 2011. The definitive version is available at http://www.sciencedirect.com

DOI: doi:10.1016/j.ijfoodmicro.2011.07.012

Abstract

This study aimed to identify factors that influence the development of biofilm by Listeria monocytogenes strains and to determine the extent to which biofilm production protects against quaternary ammonium compound (QAC) disinfectant challenge. A total of 95 L. monocytogenes strains were studied and biofilm production was assessed as a function of incubation temperature, media pH, strain origin, serotype, and environmental persistence status. Attachment and biofilm development (inferred by the level of attached biomass) were measured in vitro using a colourimetric 96-well microtitre plate method in nutritive media (Brain–Heart Infusion). Increased biofilm production correlated with increasing temperature and the most acidic, or most alkaline, growth conditions tested. Clinical and environmental (food factory) strains were observed to increase biofilm production at higher and lower incubation temperatures respectively,independent of their rate of planktonic growth. Serotype 1/2a strains produced significantly more biofilm. Biofilm maturity, rather than strain, was correlated with resistance to QAC. Carbohydrate containing exopolymeric material could not be detected in the biofilm of representative strains, and no correlation between strains recovered as persistent food factory contaminants and biofilm production was identified. Although limited to in vitro inference based on the assay system used, our results suggest that environmental conditions determine the level of biofilm production by L. monocytogenes strains, independent of the rate of planktonic growth, and that this may manifest from selection pressures to which a given strain grows optimally.

Item Details

Item Type:Refereed Article
Keywords:biofilm, environmental selection, stress, temperature, pH
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Public Health (excl. Specific Population Health) not elsewhere classified
Author:Nilsson, RE (Mr Rolf Nilsson)
Author:Ross, T (Associate Professor Tom Ross)
Author:Bowman, JP (Associate Professor John Bowman)
ID Code:63135
Year Published:2011
Web of Science® Times Cited:64
Deposited By:Agricultural Science
Deposited On:2010-04-15
Last Modified:2017-11-06
Downloads:9 View Download Statistics

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