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Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L
Citation
Taylor, RS and Crosbie, PBB and Cook, MT, Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L, Journal of Fish Diseases, 33, (1) pp. 1-14. ISSN 0140-7775 (2010) [Refereed Article]
DOI: doi:10.1111/j.1365-2761.2009.01108.x
Abstract
Abstract
Amoebic gill disease (AGD) is a proliferative gill
tissue response caused by Neoparamoeba perurans
and is the main disease affecting Australian marine
farmed Atlantic salmon. We have previously proposed
that macroscopic gill health (gill score) trajectories
and challenge survival provide evidence of
a change in the nature of resistance to AGD. In
order to examine whether the apparent development
of resistance was because of an adaptive
response, serum was sequentially sampled from the
same individuals over the first three rounds of
natural AGD infection and from survivors of a
subsequent non-intervention AGD survival challenge.
The systemic immune reaction to wildtype
Neoparamoeba sp. was characterized by Western
blot analysis and differentiated to putative carbohydrate
or peptide epitopes by periodate oxidation
reactions. The proportion of seropositive fish
increased from 46% to 77% with each AGD round.
Antibody response to carbohydrate epitope(s) was
immunodominant, occurring in 43–64% of
samples. Antibodies that bound peptide epitope
were identified in 16% of the challenge survivors. A
1:50 (single-dilution) enzyme-linked immunosorbent
assay confirmed a measurable immune titre
in 13% of the survivors. There was no evidence that
antibodies recognizing wildtype Neoparamoeba
provided significant protection against AGD.
Keywords: AGD, ectoparasite, ELISA, Neoparamoeba,
Western blot.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Fish pests and diseases |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Aquaculture fin fish (excl. tuna) |
UTAS Author: | Taylor, RS (Dr Richard Taylor) |
UTAS Author: | Crosbie, PBB (Dr Philip Crosbie) |
ID Code: | 63068 |
Year Published: | 2010 |
Web of Science® Times Cited: | 12 |
Deposited By: | NC Marine Conservation and Resource Sustainability |
Deposited On: | 2010-04-13 |
Last Modified: | 2015-02-02 |
Downloads: | 0 |
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