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Complexation of metals by phytosiderophores revealed by CE-ESI-MS and CE-ICP-MS


Dell'mour, M and Koellensperger, G and Quirino, JP and Haddad, PR and Stanetty, C and Oburger, E and Puschenreiter, M and Hann, S, Complexation of metals by phytosiderophores revealed by CE-ESI-MS and CE-ICP-MS, Electrophoresis, 31, (7) pp. 1201-1207. ISSN 0173-0835 (2010) [Refereed Article]

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DOI: doi:10.1002/elps.200900635


CE-ESI-MS and CE-ICP-MS were implemented for studying three phytosiderophores (mugineic acid, epi-mugineic acid and deoxymugineic acid) and their metal complexes. Free ligands and ferric complexes were analyzed using the first methodology, while six free metals (Co(II), Cu(II), Fe(III), Mn(II), Ni(II) and Zn(II)) together with the corresponding complexes were investigated by the latter technique. CE separation was realized at a voltage of +25 kV employing a BGE containing 20 mM ammonium bicarbonate at pH 7.2. Both techniques revealed limits of detection in the high nM to low M range. Standard additions to hydroponic samples of H. distichon, cv. Bodega (spring barley) showed regression coefficients for the metal-ligand complexes ranging from 0.984 to 0.999. Additionally, results of a competitivity study allowed the determination of relative metal-phytosiderophore complex stability constants of deoxymugineic/mugineic acid.

Item Details

Item Type:Refereed Article
Keywords:CE-ESI-MS CE-ICP-MS Metal complexation Phytosiderophores Rhizosphere
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Dell'mour, M (Ms Madeleine Dell-mour)
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:62985
Year Published:2010
Web of Science® Times Cited:32
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-03-31
Last Modified:2015-01-21
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