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Processing of complex antigens and simple hapten-like molecules by epidermal Langerhans cells


Woods, GM and Henderson, KG and Qu, MM and Muller, HK, Processing of complex antigens and simple hapten-like molecules by epidermal Langerhans cells, Leukocyte Biology, 57, (6) pp. 891-896. ISSN 0741-5400 (1995) [Refereed Article]

DOI: doi:10.1002/jlb.57.6.891


Langerhans cells (LCs) are antigen-presenting cells of the skin that trap small contact-sensitizing molecules and induce cutaneous hypersensitivity. LCs can present larger molecules but the mechanisms of processing have required investigation. A system combining in vitro culture of antigen with epidermal cells in the presence of inhibitors, followed by fixation and transfer of these antigen/drug treated epidermal cells to naive mice, was developed to investigate the steps of antigen processing. Langerhans cells undertake similar, but not identical, pathways for the processing of simple and complex molecules. Complex molecules such as trinitrophenyl conjugated to ovalbumin (TNP-OVA) were internalized Mice and modification required a chloroquine-sensitive proteolysis step and a cycloheximide-sensitive protein synthesis step. This modified product was actively recycled to the cell membrane as presentation was inhibited by blocking receptor translocation with either monensin or cytochalasin B. Small contact sensitizers such as trinitrophenyl did not undergo modification but required internalization and presentation was also inhibited by blocking receptor translocation.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Cellular immunology
Objective Division:Health
Objective Group:Other health
Objective Field:Other health not elsewhere classified
UTAS Author:Woods, GM (Professor Gregory Woods)
UTAS Author:Qu, MM (Mrs Miao Qu)
UTAS Author:Muller, HK (Professor Konrad Muller)
ID Code:6278
Year Published:1995
Web of Science® Times Cited:5
Deposited By:Pathology
Deposited On:1995-08-01
Last Modified:2011-08-25

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