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Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle

Citation

Clerk, LH and Vincent, MA and Jahn, LA and Liu, Z and Lindner, JR and Barrett, EJ, Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle , Diabetes, 55, (5) pp. 1436-1442. ISSN 0012-1797 (2006) [Refereed Article]

DOI: doi:10.2337/db05-1373

Abstract

We have previously shown that skeletal muscle capillaries are rapidly recruited by physiological doses of insulin in both humans and animals. This facilitates glucose and insulin delivery to muscle, thus augmenting glucose uptake. In obese rats, both insulin-mediated microvascular recruitment and glucose uptake are diminished; however, this action of insulin has not been studied in obese humans. Here we used contrast ultrasound to measure microvascular blood volume (MBV) (an index of microvascular recruitment) in the forearm flexor muscles of lean and obese adults before and after a 120-min euglycemic-hyperinsulinemic (1 mUmin-1kg -1) clamp. We also measured brachial artery flow, fasting lipid profile, and anthropomorphic variables. Fasting plasma glucose (5.4 0.1 vs. 5.1 0.1 mmol/l, P = 0.05), insulin (79 11 vs. 38 6 pmol/l, P = 0.003), and percent body fat (44 2 vs. 25 2%, P = 0.001) were higher in the obese than the lean adults. After 2 h of insulin infusion, whole-body glucose infusion rate was significantly lower in the obese versus lean group (19.3 3.2 and 37.4 2.6 mol min-1kg-1 respectively, P < 0.001). Compared with baseline, insulin increased MBV in the lean (18.7 3.3 to 25.0 4.1, P = 0.019) but not in the obese group (20.4 3.6 to 18.8 3.8, NS). Insulin increased brachial artery diameter and flow in the lean but not in the obese group. We observed a significant, negative correlation between MBV and BMI (R = -0.482, P = 0.027) in response to insulin. In conclusion, obesity eliminated the insulin-stimulated muscle microvascular recruitment and increased brachial artery blood flow seen in lean individuals.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Endocrinology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Diabetes
Author:Vincent, MA (Dr Michelle Keske)
ID Code:62766
Year Published:2006
Web of Science® Times Cited:161
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-03-22
Last Modified:2010-05-03
Downloads:0

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