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Contraction stimulates nitric oxide independent microvascular recruitment and increases muscle insulin uptake
Citation
Inyard, AC and Clerk, LH and Vincent, MA and Barrett, EJ, Contraction stimulates nitric oxide independent microvascular recruitment and increases muscle insulin uptake , Diabetes, 56, (9) pp. 2194-2200 . ISSN 0012-1797 (2007) [Refereed Article]
Abstract
We examined whether contraction-induced muscle microvascular recruitment would expand the surface area for insulin and nutrient exchange and thereby contribute to insulin-mediated glucose disposal. We measured in vivo rat hindlimb microvascular blood volume (MBV) using contrast ultrasound and femoral blood flow (FBF) using Doppler ultrasound in response to a stimulation frequency range. Ten minutes of 0.1-Hz isometric contraction more than doubled MBV (P < 0.05; n = 6) without affecting FBF (n = 7), whereas frequencies >0.5 Hz increased both. Specific inhibition of nitric oxide (NO) synthase with N¥ø-L-nitro-arginine-methyl ester (n = 5) significantly elevated mean arterial pressure by ¡30 mmHg but had no effect on basal FBF or MBV. We next examined whether selectively elevating MBV without increasing FBF (0.1-Hz contractions) increased muscle uptake of albumin-bound Evans blue dye (EBD). Stimulation at 0.1 Hz (10 min) elicited more than twofold increases in EBD content (micrograms EBD per gram dry tissue) in stimulated versus contralateral muscle (n = 8; 52.2 ¡¾ 3.8 vs. 20 ¡¾ 2.5, respectively; P < 0.001). We then measured muscle uptake of EBD and 125I-labeled insulin (dpm per gram dry tissue) with 0.1-Hz stimulation (n = 6). Uptake of EBD (19.1 ¡¾ 3.8 vs. 9.9 ¡¾ 1; P < 0.05) and 125I-insulin (5,300 ¡¾ 800 vs. 4,244 ¡¾ 903; P < 0.05) was greater in stimulated muscle versus control. Low-frequency contraction increases muscle MBV by a NO-independent pathway and facilitates muscle uptake of albumin and insulin in the absence of blood flow increases. This microvascular response may, in part, explain enhanced insulin action in exercising skeletal muscle.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Clinical sciences |
| Research Field: | Endocrinology |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | Vincent, MA (Dr Michelle Keske) |
| ID Code: | 62763 |
| Year Published: | 2007 |
| Web of Science® Times Cited: | 62 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2010-03-22 |
| Last Modified: | 2010-05-03 |
| Downloads: | 0 |
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