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Micro-bore titanium housed polymer monoliths for reversed-phase liquid chromatography of small molecules


Nesterenko, EP and Nesterenko, PN and Connolly, D and Lacroix, F and Paull, B, Micro-bore titanium housed polymer monoliths for reversed-phase liquid chromatography of small molecules, The Journal of Chromatography A, 1217, (14) pp. 2138-2146. ISSN 0021-9673 (2010) [Refereed Article]

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DOI: doi:10.1016/j.chroma.2010.02.007


A new method for the fixation of polymethacrylate monoliths within titanium tubing of up to 0.8 mm I.D. for use as a chromatographic column under elevated temperatures and pressures is described. The preparation of butyl methacrylate�ethylene dimethacrylate-based monolithic stationary phases with desired porous structures was achieved within titanium tubing with pre-oxidised internal walls. The oxidised titanium surface was subsequently silanised with 3-trimethoxysilylpropyl methacrylate resulting in tight bonding of butyl methacrylate porous monolith to the internal walls, providing stationary phase stability at column temperatures up to 110 �C and at operating column pressure drops of >28 MPa. The titanium housed monoliths exhibited a uniform and dense porous structure, which provided peak efficiencies of up to 59,000 theoretical plates per meter when evaluated for the separation of small molecules in reversed-phase mode, under optimal conditions (achieved at 15 �L/min and temperature of 110 �C for naphthalene with a retention factor, k = 0.58). The developed column was applied to the reversed-phase isocratic separation of a text mixture of pesticides.

Item Details

Item Type:Refereed Article
Keywords:Titanium column; Polymer monolith; High pressure reversed-phase HPLC
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Nesterenko, PN (Professor Pavel Nesterenko)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:62678
Year Published:2010
Web of Science® Times Cited:29
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-03-17
Last Modified:2015-01-20
Downloads:1 View Download Statistics

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