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Development of microsatellite markers for the short-beaked echidna using three different approaches


Vanpe, C and Buschiazzo, E and Abdelkrim, J and Morrow, GE and Nicol, SC and Gemmell, NJ, Development of microsatellite markers for the short-beaked echidna using three different approaches, Australian Journal of Zoology, 57, (4) pp. 219-224. ISSN 0004-959X (2009) [Refereed Article]

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Copyright © 2009 CSIRO

DOI: doi:10.1071/ZO09033


Abstract. We used three different methods, size-selected genomic library, cross-species amplification of a mammal-wide set of conserved microsatellites and genomic sequencing, to develop a panel of 43 microsatellite loci for the short-beaked echidna (Tachyglossus aculeatus). These loci were screened against 13 individuals from three different regions (Tasmania, Kangaroo Island, Perth region), spanning the breadth of the range of the short-beaked echidna. Nine of the 43 tested loci amplified reliably, generated clear peaks on the electropherogram and were polymorphic, with the number of alleles per locus ranging from two to eight (mean = 3.78) in the individuals tested. Polymorphic information content ranged from 0.16 to 0.78, and expected heterozygosity ranged from 0.19 to 0.84. One of the nine microsatellites showed a heterozygote deficit, suggesting a high probability of null alleles. The genomic sequencing approach using data derived from the Roche FLX platform is likely to provide the most promising method to develop echidna microsatellites. The microsatellite markers developed here will be useful tools to study population genetic structure, gene flow, kinship and parentage in Tachyglossus sp. and potentially also in endangered Zaglossus species.

Item Details

Item Type:Refereed Article
Keywords:454 sequencing, cross-species amplification, echidna, genomic library, microsatellite, monotremes, Tachyglossus aculeatus
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal physiological ecology
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Terrestrial biodiversity
UTAS Author:Morrow, GE (Ms Gemma Morrow)
UTAS Author:Nicol, SC (Associate Professor Stewart Nicol)
ID Code:62176
Year Published:2009
Web of Science® Times Cited:9
Deposited By:Zoology
Deposited On:2010-03-10
Last Modified:2010-04-27
Downloads:2 View Download Statistics

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