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New lv mutants of pea are deficient in phytochrome B


Weller, JL and Nagatani, A and Kendrick, RE and Murfet, IC and Reid, JB, New lv mutants of pea are deficient in phytochrome B, Plant Physiology, 108, (2) pp. 525-532. ISSN 0032-0889 (1995) [Refereed Article]

DOI: doi:10.1104/pp.108.2.525


The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three fur-ther lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable from wild type under far-red light. The phyB apoprotein present in the lv-1 mutant was undetectable in all three new lv mutants. The identification of allelic mutants with and without phyB apoprotein suggests that Lv may be a structural gene for a B-type phytochrome. Furthermore, it indicates that the lv-1 mutation results specifically in the loss of normal biological activity of this phytochrome. Red-light-pulse and fluence-rate-response experiments suggest that lv plants are deficient in the low-fluence response (LFR) but retain a normal very-low-fluence-rate-dependent response for leaflet expansion and inhibition of stem elongation. Comparison of lv alleles of differing severity indicates that the LFR for stem elongation can be mediated by a lower level of phyB than the LFR for leaflet expansion. The retention of a strong response to continuous low-fluence-rate R in all four lv mutants suggests that there may be an additional phytochrome controlling responses to R in pea. The kinetics of phytochrome destruction and reaccumulation in the lv mutant indicate that phyB may be involved in the light regulation of phyA levels.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biotechnology
Research Field:Medical biotechnology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Weller, JL (Associate Professor Jim Weller)
UTAS Author:Nagatani, A ( Nagatani)
UTAS Author:Kendrick, RE ( Kendrick)
UTAS Author:Murfet, IC (Professor Ian Murfet)
UTAS Author:Reid, JB (Professor Jim Reid)
ID Code:6207
Year Published:1995
Web of Science® Times Cited:58
Deposited By:Plant Science
Deposited On:1995-08-01
Last Modified:2010-11-08

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