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Antioxidants: Promising neuroprotection against cardiotoxin-4b-induced cell death which triggers oxidative stress with early calpain activation
Citation
Tan, TB and Chen, MJ and Armugam, A and Peng, ZF and Li, QT and Jeyaseelan, K and Cheung, NS, Antioxidants: Promising neuroprotection against cardiotoxin-4b-induced cell death which triggers oxidative stress with early calpain activation, Toxicon: An Interdisciplinary Journal on The Toxins Derived From Animals, Plants and Microorganisms, 51, (6) pp. 964-973. ISSN 0041-0101 (2008) [Refereed Article]
DOI: doi:10.1016/j.toxicon.2007.11.019
Abstract
Cardiotoxin-4b (CTX-4b), isolated from Naja naja sputatrix venom, shows lethality in several cell types. Employing murine primary cortical neurons, this study was undertaken to investigate the molecular mechanisms of CTX-4b in the induction of neuronal death. CTX-4b induced a dose- and time-dependent neuronal death. Strong induction of calpains as early as 4 h post-CTX-4b 75 nM treatment was detected in neurons with negligible caspase 3 activation. For the first time in cultured murine primary cortical neurons, it was noted that CTX-4b-mediated cell death triggered oxidative stress with an increase in reactive oxygen species (ROS) levels, and that application of antioxidants showed effective attenuation of cell death. Taken together, these results indicate that CTX-4b-mediated neuronal death is associated with (i) early calpain activation and (ii) oxidative stress. Most importantly, antioxidants have proved to be a promising therapeutic avenue against CTX-4b-induced neuronal death.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biomedical and Clinical Sciences |
Research Group: | Neurosciences |
Research Field: | Cellular nervous system |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Cheung, NS (Dr Nam Cheung) |
ID Code: | 61648 |
Year Published: | 2008 |
Web of Science® Times Cited: | 5 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2010-03-04 |
Last Modified: | 2010-05-04 |
Downloads: | 0 |
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