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Microtubule association of the neuronal p35 activator of Cdk5
Citation
Hou, Z and Li, Q and He, L and Lim, HY and Fu, X and Cheung, NS and Qi, DX and Qi, RZ, Microtubule association of the neuronal p35 activator of Cdk5, Journal of Biological Chemistry, 282, (26) pp. 18666-18670. ISSN 0021-9258 (2007) [Refereed Article]
DOI: doi:10.1074/jbc.C700052200
Abstract
Cdk5 and its neuronal activator p35 play an important role in neuronal migration and proper development of the brain cortex. We show that p35 binds directly to á/â-tubulin and microtubules. Microtubule polymers but not the á/â-tubulin heterodimer block p35 interaction with Cdk5 and therefore inhibit Cdk5-p35 activity. p25, a neurotoxin-induced and truncated form of p35, does not have tubulin and microtubule binding activities, and Cdk5-p25 is inert to the inhibitory effect of microtubules. p35 displays strong activity in promoting microtubule assembly and inducing formation of microtubule bundles. Furthermore, microtubules stabilized by p35 are resistant to cold-induced disassembly. In cultured cortical neurons, a significant proportion of p35 localizes to microtubules. When microtubules were isolated from rat brain extracts, p35 co-assembled with microtubules, including cold-stable microtubules. Together, these findings suggest that p35 is a microtubule-associated protein that modulates microtubule dynamics. Also, microtubules play an important role in the control of Cdk5 activation.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biomedical and Clinical Sciences |
Research Group: | Neurosciences |
Research Field: | Cellular nervous system |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Cheung, NS (Dr Nam Cheung) |
ID Code: | 61630 |
Year Published: | 2007 |
Web of Science® Times Cited: | 32 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2010-03-04 |
Last Modified: | 2010-05-04 |
Downloads: | 0 |
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