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Identification of nuclear import mechanisms for the neuronal Cdk5 activator
Citation
Fu, X and Choi, YK and Qu, D and Yu, Y and Cheung, NS and Qi, RZ, Identification of nuclear import mechanisms for the neuronal Cdk5 activator, Journal of Biological Chemistry, 281, (51) pp. 39014-39021. ISSN 0021-9258 (2006) [Refereed Article]
DOI: doi:10.1074/jbc.M512663200
Abstract
The activation of Cdk5 by p35 plays a pivotal role in a multitude of nervous system activities ranging from neuronal differentiation to degeneration. A fraction of Cdk5 and p35 localizes in the nucleus where Cdk5-p35 exerts its functions via protein phosphorylation, and p35 displays a dynamic localization between the cytoplasm and the nucleus. Here, we examined the nuclear import properties of p35. In nuclear import assays, p35 was actively transported into the nuclei of digitonin-permeabilized HeLa cells and cortical neurons by cytoplasmic carrier-mediated mechanisms. Importin-â, importin-5, and importin-7 were identified to import p35 into the nuclei via a direct interaction with it. An N-terminal region of p35 was defined to interact with the above importins, serving as a nuclear localization signal. Finally, we show that the nuclear localization of p35 does not require the association of Cdk5. Furthermore, Cdk5 and importin-â/5/7 are mutually exclusive in binding to p35. These results suggest that p35 employs pathways distinct from that used by Cdk5 for transport to the nucleus.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biomedical and Clinical Sciences |
Research Group: | Neurosciences |
Research Field: | Cellular nervous system |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Cheung, NS (Dr Nam Cheung) |
ID Code: | 61624 |
Year Published: | 2006 |
Web of Science® Times Cited: | 43 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2010-03-04 |
Last Modified: | 2010-05-04 |
Downloads: | 0 |
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