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Surface Plasmon Resonance for the Analysis of β-Amyloid Interactions and Fibril Formation in Alzheimer's Disease Research

journal contribution
posted on 2023-05-17, 01:39 authored by Aguilar, MI, David SmallDavid Small
Alzheimer's disease (AD) is a neurodegenerative disorder characterised by the accumulation of amyloid deposits, the major component of which is a 4 kDa polypeptide known as â-amyloid protein (Aá). Identifying the mechanism underlying the formation of Aâ and the pathways that lead to its toxicity is crucial to understanding the mechanism of AD and addressing the urgent need for new and effective treatments for AD. The accumulation of Aâ is the result of a complex interplay between genetic and environmental factors that affect the generation, clearance and aggregation of the peptide. Because of its propensity to aggregate, Aâ builds up in the brain and assembles into amyloid fibrils, ultimately creating amyloid plaques (APs) and cerebral amyloid angiopathy (CAA). Aâ has been shown to interact with a number of intracellular and extracellular molecules, but the relative contribution of these interactions to the toxicity of Aâ is not well understood. A critical step in characterising the importance of these interactions is the ability to measure both the affinity and kinetics of these interactions. Surface plasmon resonance (SPR) spectroscopy has become a widely used technique to study molecular interactions such as antibody-antigen, DNA-DNA, DNA-protein, protein-protein, receptor-ligand and peptide- and protein-membrane interactions. This article reviews the application of SPR to the study of the molecular interactions associated with AD and how this information enhances our molecular understanding of Aâ-mediated toxicity

History

Publication title

Neurotoxicity Research: An International Journal on Processes and Mechanisms in Neurodegeneration, Apoptosis, Neuroprotection and Regeneration

Volume

7

Issue

1-2

Pagination

17-27

ISSN

1029-8428

Department/School

Menzies Institute for Medical Research

Publisher

F P Graham Publishing Co

Place of publication

Po Box 370, Mountain Home, USA, Tn, 37684

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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