Objective: Noradrenaline treatment of animals results in postnatal hypertrophy of the heart. This process requires many qualitative and quantitative changes in gene expression; however, the identities of the key regulatory genes which modulate the process are not known. The aim of this study was to investigate whether a recently developed technique, differential display, could provide a new route to the identification and characterisation of these critical genes. Methods: The technique of differential display was modified for use on cardiac RNA samples and the expression of clones identified by this approach was characterised by northern analysis. Results: Differential display was successfully adapted to the study of noradrenaline induced cardiac gene expression. A previously unsuspected gene was identified, the expression of which appears to be strongly modified during the onset of this process. Conclusions: Differential display offers the potential to identify and clone many of the genes critically important in regulation of growth of the mammalian heart.

Item Type:	Refereed Article
Research Division:	Biological Sciences
Research Group:	Biochemistry and Cell Biology
Research Field:	Cell Metabolism
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Environmental Sciences
Author:	Venkataraman, R (Mr Rajan Venkataraman)
Author:	Presser, JL (Ms Jennifer Louise Presser)
Author:	Vaillancourt, RE (Professor Rene Vaillancourt)
Author:	West, AK (Professor Adrian West)
ID Code:	6126
Year Published:	1995
Web of Science® Times Cited:	4
Deposited By:	Biochemistry
Deposited On:	1995-08-01
Last Modified:	2011-08-25
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