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Identification of a new noradrenaline induced gene in the rat heart by differential messenger-RNA display


Venkataraman, R and Presser, JL and Vaillancourt, RE and West, AK, Identification of a new noradrenaline induced gene in the rat heart by differential messenger-RNA display, Cardiovascular Research, 29, (4) pp. 490-494. ISSN 0008-6363 (1995) [Refereed Article]

DOI: doi:10.1016/0008-6363(96)88524-5


Objective: Noradrenaline treatment of animals results in postnatal hypertrophy of the heart. This process requires many qualitative and quantitative changes in gene expression; however, the identities of the key regulatory genes which modulate the process are not known. The aim of this study was to investigate whether a recently developed technique, differential display, could provide a new route to the identification and characterisation of these critical genes. Methods: The technique of differential display was modified for use on cardiac RNA samples and the expression of clones identified by this approach was characterised by northern analysis. Results: Differential display was successfully adapted to the study of noradrenaline induced cardiac gene expression. A previously unsuspected gene was identified, the expression of which appears to be strongly modified during the onset of this process. Conclusions: Differential display offers the potential to identify and clone many of the genes critically important in regulation of growth of the mammalian heart.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell metabolism
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Venkataraman, R (Mr Rajan Venkataraman)
UTAS Author:Presser, JL (Ms Jennifer Louise Presser)
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
UTAS Author:West, AK (Professor Adrian West)
ID Code:6126
Year Published:1995
Web of Science® Times Cited:4
Deposited By:Biochemistry
Deposited On:1995-08-01
Last Modified:2011-08-25

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