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Nitrogen excretion by the sheep abomasal parasite Teladorsagia circumcincta

journal contribution
posted on 2023-05-17, 01:34 authored by Simpson, HV, Muhamad, N, Walker, LR, Simcock, DC, Brown, SC, Pedley, KC
Excretion of nitrogenous substances by Teladorsagia circumcincta was investigated during incubation of L3 in phosphate buffer for up to 30 h and adult worms for 4-6 h. Ammonia was the main excretory product, with about 20% urea. For the first 4-6 h, ammonia excretion by L3 was temperature dependent, directly proportional to the number of larvae, but independent of the pH or strength of the phosphate buffer. Later, ammonia excretion slowed markedly in L3 and adults and reversed to net uptake in L3 by 30 h. An initial external ammonia concentration of 600 μM did not alter the pattern or magnitude of excretion. Re-uptake of ammonia did not occur at extremes of pH or low buffer strength and was slightly reduced at the highest external concentrations. Ammonium transporters and enzymes of glutamate metabolism, including glutamate dehydrogenase, glutamine synthetase and possibly glutamate synthase, are worthy of further investigation as anthelmintic targets. © 2009 Elsevier Inc. All rights reserved.

History

Publication title

Experimental Parasitology

Volume

123

Pagination

17-23

ISSN

0014-4894

Department/School

School of Health Sciences

Publisher

Academic Press Inc Elsevier Science

Place of publication

525 B St, Ste 1900, San Diego, USA, Ca, 92101-4495

Repository Status

  • Restricted

Socio-economic Objectives

Sheep for wool

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