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Quantitative determination of glucoraphanin in Brassica vegetables by micellar electrokinetic capillary chromatography

Citation

Lee, I and Boyce, MC and Breadmore, MC, Quantitative determination of glucoraphanin in Brassica vegetables by micellar electrokinetic capillary chromatography , Analytica Chimica Acta, 663, (1) pp. 105-108. ISSN 0003-2670 (2010) [Refereed Article]


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DOI: doi:10.1016/j.aca.2010.01.043

Abstract

Glucoraphanin, a glucosinolate, is found naturally in plants and is present in relatively high concentrations in broccoli. Glucosinolates have received much attention as studies have indicated that a diet rich in them may provide some protection from certain cancers. A micellar electrokinetic chromatography (MEKC) method using sodium cholate as the micellar phase has been developed to quantify for glucoraphanin in broccoli (seeds and florets) and Brussels sprouts. The glucoraphanin peak elutes just under 5 min with a theoretical plate number of 380,000 per metre of capillary. The method is suitable for crude extracts of broccoli and Brussels sprouts. Glucoraphanin in broccoli seeds (1330 mg/100 g) broccoli florets (89 mg/100 g) and Brussels sprouts (3 mg/100 g) was determined and agreed with the data obtained by high performance liquid chromatography. The LODs were 10100 times below the levels typically found in broccoli seeds (4 mg/100 g), broccoli florets (0.9 mg/100 g) and Brussels sprouts (0.1 mg/100 g).

Item Details

Item Type:Refereed Article
Keywords:Broccoli; Sodium cholate; Capillary electrophoresis
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:60985
Year Published:2010
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:8
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-02-24
Last Modified:2011-05-04
Downloads:3 View Download Statistics

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