eCite Digital Repository

Effective reversed-phase ion pair high-performance liquid chromatography method for the separation and characterization of intact low-molecular-weight heparins

Citation

Patel, R and Narkowicz, CK and Jacobson, GA, Effective reversed-phase ion pair high-performance liquid chromatography method for the separation and characterization of intact low-molecular-weight heparins , Analytical Biochemistry, 387, (1) pp. 113-121. ISSN 0003-2697 (2009) [Refereed Article]


Preview
PDF
Restricted - Request a copy
844Kb
  

Copyright Statement

The definitive version is available at http://www.sciencedirect.com

Official URL: http://www.sciencedirect.com

DOI: doi:10.1016/j.ab.2009.01.007

Abstract

A simple, selective, and efficient reversed-phase ion pair high-performance liquid chromatography (RPIP-HPLC) method was developed for the separation of various commercially available intact low-molecular-weight heparins (LMWHs). The developed method uses a C18 column (150 4.6 mm) with diode array detection at 230 nm, flow rate at 1.0 ml/min, and a mobile phase containing acetonitrile/water (32:68%), tetrabutylammonium hydroxide (15 mM), and ammonium acetate (50 mM) at pH 7.0. The performance of this method was assessed in terms of selectivity, linearity, intra- and interday precision, and accuracy. The novel application of RPIP-HPLC with evaporative light scattering detection (ELSD) for the analysis of intact LMWHs was demonstrated. Intact LMWHs were analyzed with superior resolution and peak shape. Different chromatographic profiles were obtained for different LMWHs showing significant structural diversity. This method clearly showed chemical changes that occurred to LMWH under the stress condition. This method can be applied for the separation, identification, characterization, and pharmaceutical stability analysis of various LMWHs.

Item Details

Item Type:Refereed Article
Keywords:reversed-phase ion pair,liquid chromatography, low-molecular-weight heparins
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Pharmaceutical Sciences
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
Author:Patel, R (Dr Rahul Patel)
Author:Narkowicz, CK (Dr Christian Narkowicz)
Author:Jacobson, GA (Dr Glenn Jacobson)
ID Code:60661
Year Published:2009
Web of Science® Times Cited:30
Deposited By:Pharmacy
Deposited On:2010-02-15
Last Modified:2011-02-19
Downloads:1 View Download Statistics

Repository Staff Only: item control page