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DIE NEUTRALIS and LATE BLOOMER 1 Contribute to Regulation of the Pea Circadian Clock


Liew, LC and Hecht, VFG and Laurie, RE and Knowles, CL and Vander Schoor, JK and Macknight, RC and Weller, JL, DIE NEUTRALIS and LATE BLOOMER 1 Contribute to Regulation of the Pea Circadian Clock , Plant Cell, 21, (10) pp. 3198-3211. ISSN 1040-4651 (2009) [Refereed Article]

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2009 American Society of Plant Biologists

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DOI: doi:10.1105/tpc.109.067223


The DIE NEUTRALIS (DNE) locus in garden pea (Pisum sativum) was previously shown to inhibit flowering under noninductive short-day conditions and to affect a graft-transmissible flowering signal. In this study, we establish that DNE has a role in diurnal and/or circadian regulation of several clock genes, including the pea GIGANTEA (GI) ortholog LATE BLOOMER 1 (LATE1) and orthologs of the Arabidopsis thaliana genes LATE ELONGATED HYPOCOTYL and TIMING OF CHLOROPHYLL A/B BINDING PROTEIN EXPRESSION 1. We also confirm that LATE1 participates in the clock and provide evidence that DNE is the ortholog of Arabidopsis EARLY FLOWERING4 (ELF4). Circadian rhythms of clock gene expression in wild-type plants under constant light were weaker in pea than in Arabidopsis, and a number of differences were also seen in the effects of both DNE/ELF4 and LATE1/GI on clock gene expression. Grafting studies suggest that DNE controls flowering at least in part through a LATE1-dependent mobile stimulus, and dne mutants show elevated expression of a FLOWERING LOCUS T homolog under short-day conditions. However, the early flowering of the dne mutant is not associated with altered expression of a previously described CONSTANS-like gene. Collectively, our results characterize the clock system and reveal its importance for photoperiod responsiveness in a model legume.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Grain legumes
UTAS Author:Liew, LC (Miss Lim Chee Liew)
UTAS Author:Hecht, VFG (Dr Valerie Hecht)
UTAS Author:Knowles, CL (Dr Claire Knowles)
UTAS Author:Vander Schoor, JK (Mrs Jacqueline Vander Schoor)
UTAS Author:Weller, JL (Associate Professor Jim Weller)
ID Code:60657
Year Published:2009
Web of Science® Times Cited:51
Deposited By:Plant Science
Deposited On:2010-02-15
Last Modified:2015-02-04

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