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Epidemiology of Pseudomonas aeruginosa in a tertiary referral teaching hospital
Citation
Bradbury, RS and Champion, AC and Reid, DW, Epidemiology of Pseudomonas aeruginosa in a tertiary referral teaching hospital , Journal of Hospital Infection, 73, (2) pp. 151-156. ISSN 0195-6701 (2009) [Refereed Article]
DOI: doi:10.1016/j.jhin.2009.05.021
Abstract
A genotypically indistinguishable strain of Pseudomonas aeruginosa (Australian epidemic strain III: AES III) has previously been found in a proportion of adults with cystic fibrosis (CF) in Tasmania, Australia. The aim of this study was to identify a source of these infections within the major tertiary referral hospital for the State of Tasmania, and to determine if this strain could be isolated from settings other than the CF lung. A total of 120 isolates of P. aeruginosa were collected from clinical and environmental sources within the hospital and from environmental locations in the hospital vicinity. These isolates were genotyped by random amplification of polymorphic DNA (RAPD)–polymerase chain reaction (PCR) and antimicrobial susceptibility testing was performed using the Clinical and Laboratory Standards Institute method. Confirmation of similar genotypes identified by RAPD–PCR was performed using pulsed-field gel electrophoresis with restriction enzyme SpeI. AES III was not recovered from any source other than the respiratory secretions of CF patients. P. aeruginosa in the non-CF settings was found to be panmictic, and no cross-infection or acquisition of hospital environment strains by patients was observed.
Item Details
Item Type: | Refereed Article |
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Keywords: | Cystic fibrosis; Epidemiology; Hospital; Molecular epidemiology; Pseudomonas aeruginosa |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Medical microbiology |
Research Field: | Medical bacteriology |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Bradbury, RS (Dr Richard Bradbury) |
UTAS Author: | Champion, AC (Dr Alan Champion) |
UTAS Author: | Reid, DW (Dr David Reid) |
ID Code: | 60555 |
Year Published: | 2009 |
Web of Science® Times Cited: | 11 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2010-02-11 |
Last Modified: | 2010-04-14 |
Downloads: | 0 |
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