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Hydrogen/deuterium exchange on aromatic rings during atmospheric pressure chemical ionization mass spectrometry
journal contribution
posted on 2023-05-17, 01:18 authored by Noel DaviesNoel Davies, Jason SmithJason Smith, Molesworth, PP, John RossJohn RossIt was demonstrated that substituted indoles fully labelled with deuterium on the aromatic ring can undergo substantial exchange back to partial and even fully protonated forms during Atmospheric Pressure Chemical Ionisation (APCI) LC-MS. The degree of this exchange was strongly dependent on the absolute quantity of analyte, the APCI desolvation temperature, the nature of the mobile phase, the mobile phase flow rate and the instrument used. H-D exchange on several other aromatic ring systems during APCI LC-MS was either undetectable (nitrobenzene, aniline) or extremely small (acetanilide) compared to the effect observed for substituted indoles. This observation has major implications for quantitative assays using deuterium labelled internal standards and for the detection of deuterium labelled products from isotopically labelled feeding experiments where there is a risk of back exchange to the protonated form during the analysis.
History
Publication title
Rapid Communications in Mass SpectrometryVolume
24Issue
7Pagination
1105-1110ISSN
1097-0231Department/School
School of Natural SciencesPublisher
John Wiley & SonsPlace of publication
New YorkRights statement
The definitive published version is available online at: http://interscience.wiley.comRepository Status
- Restricted