eCite Digital Repository
Hydrogen/deuterium exchange on aromatic rings during atmospheric pressure chemical ionization mass spectrometry
Citation
Davies, NW and Smith, JA and Molesworth, PP and Ross, JJ, Hydrogen/deuterium exchange on aromatic rings during atmospheric pressure chemical ionization mass spectrometry , Rapid Communications in Mass Spectrometry, 24, (7) pp. 1105-1110. ISSN 1097-0231 (2010) [Refereed Article]
![]() | PDF Restricted - Request a copy 112Kb |
Copyright Statement
The definitive published version is available online at: http://interscience.wiley.com
Official URL: http://interscience.wiley.com
DOI: doi:10.1002/rcm.4488
Abstract
It was demonstrated that substituted indoles fully labelled with deuterium on the aromatic ring can undergo substantial exchange back to partial and even fully protonated forms during Atmospheric Pressure Chemical Ionisation (APCI) LC-MS. The degree of this exchange was strongly dependent on the absolute quantity of analyte, the APCI desolvation temperature, the nature of the mobile phase, the mobile phase flow rate and the instrument used. H-D exchange on several other aromatic ring systems during APCI LC-MS was either undetectable (nitrobenzene, aniline) or extremely small (acetanilide) compared to the effect observed for substituted indoles. This observation has major implications for quantitative assays using deuterium labelled internal standards and for the detection of deuterium labelled products from isotopically labelled feeding experiments where there is a risk of back exchange to the protonated form during the analysis.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | mass spectrometry, isotope dilution analysis, H-D exchange, APCI |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Instrumental methods (excl. immunological and bioassay methods) |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Davies, NW (Associate Professor Noel Davies) |
UTAS Author: | Smith, JA (Professor Jason Smith) |
UTAS Author: | Molesworth, PP (Dr Peter Molesworth) |
UTAS Author: | Ross, JJ (Associate Professor John Ross) |
ID Code: | 60553 |
Year Published: | 2010 |
Web of Science® Times Cited: | 26 |
Deposited By: | Central Science Laboratory |
Deposited On: | 2010-02-11 |
Last Modified: | 2015-02-13 |
Downloads: | 1 View Download Statistics |
Repository Staff Only: item control page