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It was demonstrated that substituted indoles fully labelled with deuterium on the aromatic ring can undergo substantial exchange back to partial and even fully protonated forms during Atmospheric Pressure Chemical Ionisation (APCI) LC-MS. The degree of this exchange was strongly dependent on the absolute quantity of analyte, the APCI desolvation temperature, the nature of the mobile phase, the mobile phase flow rate and the instrument used. H-D exchange on several other aromatic ring systems during APCI LC-MS was either undetectable (nitrobenzene, aniline) or extremely small (acetanilide) compared to the effect observed for substituted indoles. This observation has major implications for quantitative assays using deuterium labelled internal standards and for the detection of deuterium labelled products from isotopically labelled feeding experiments where there is a risk of back exchange to the protonated form during the analysis.

Item Type:	Refereed Article
Keywords:	mass spectrometry, isotope dilution analysis, H-D exchange, APCI
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Instrumental methods (excl. immunological and bioassay methods)
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Davies, NW (Associate Professor Noel Davies)
UTAS Author:	Smith, JA (Professor Jason Smith)
UTAS Author:	Molesworth, PP (Dr Peter Molesworth)
UTAS Author:	Ross, JJ (Associate Professor John Ross)
ID Code:	60553
Year Published:	2010
Web of Science® Times Cited:	26
Deposited By:	Central Science Laboratory
Deposited On:	2010-02-11
Last Modified:	2015-02-13
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