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Kbg and Kv1.3 channels mediate potassium efflux in the early phase of apoptosis in Jurkat T lymphocytes


Valencia-Cruz, G and Shabala, Lana and Delgado-Enciso, I and Shabala, SN and Bonales-Alatorree, E and Pottosin, I and Dobrovinskaya, OR, Kbg and Kv1.3 channels mediate potassium efflux in the early phase of apoptosis in Jurkat T lymphocytes, American Journal of Physiology: Cell Physiology, 297, (6) pp. C1544-C1553. ISSN 0363-6143 (2009) [Refereed Article]

DOI: doi:10.1152/ajpcell.00064.2009


Microelectrode ion flux estimation (MIFE) and patch-clamp techniques were combined for noninvasive K+ flux measurements and recording of activities of the dominant K+ channels in the early phases of apoptosis in Jurkat cells. Staurosporine (STS, 1 μM) evoked rapid (peaking around 15 min) transient K+ efflux, which then gradually decreased. This transient K+ efflux occurred concurrently with the transient increase of the K+ background (Kbg) TWIK-related spinal cord K + channel-like current density, followed by a drastic decrease and concomitant membrane depolarization. The Kv1.3 current density remained almost constant. Kv1.3 activation was not altered by STS, whereas the inactivation was shifted to more positive potentials. Contribution of Kbg and Kv1.3 channels to the transient and posttransient STS-induced K+ efflux components, respectively, was confirmed by the effects of bupivacaine, predominantly blocking Kbg current, and the Kv1.3-specific blocker margatoxin. Channel-mediated K+ efflux provoked a substantial cellular shrinkage and affected the activation of caspases. Copyright © 2009 the American Physiological Society.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal physiology - cell
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Shabala, Lana (Associate Professor Lana Shabala)
UTAS Author:Shabala, SN (Professor Sergey Shabala)
UTAS Author:Pottosin, I (Professor Igor Pottosin)
ID Code:60263
Year Published:2009
Web of Science® Times Cited:36
Deposited By:Agricultural Science
Deposited On:2010-01-27
Last Modified:2012-03-05

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