eCite Digital Repository

Using excised leaves to screen lucerne for salt tolerance


Smethurst, C and Gill, WM and Shabala, SN, Using excised leaves to screen lucerne for salt tolerance, Plant Signaling & Behaviour, 4, (1) pp. 39-41. ISSN 1559-2316 (2009) [Refereed Article]

DOI: doi:10.1071/FP08030


Salinity affects many physiological processes at all levels of plant structural organization. Being a physiologically and genetically complex trait, salinity tolerance implies a coordinated contribution of multiple mechanisms, making plant screening for salt tolerance extremely difficult. In this work, we show how the use of excised leaves can fulfill that task. We argue that, by adding NaCl directly to the transpiration stream, the protective effects of several mechanisms regulating Na+ delivery to the shoot are eliminated, enhancing PSII exposure to salinity treatment and resulting in a significant decline in leaf photochemistry (Fv/Fm characteristics). We suggest that measuring Fv/Fm characteristics on excised salt-treated leaves provides an opportunity to evaluate the efficiency of vacuolar Na+ compartmentation, arguably the most important feature for salt tolerance. We also explain the observed decline in Fv/Fm values as salt-induced structural damage to chloroplasts caused by oxidative stress. 2009 Landes Bioscience.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Smethurst, C (Dr Christiane Smethurst)
UTAS Author:Gill, WM (Dr Warwick Gill)
UTAS Author:Shabala, SN (Professor Sergey Shabala)
ID Code:60243
Year Published:2009
Deposited By:Agricultural Science
Deposited On:2010-01-25
Last Modified:2012-02-09

Repository Staff Only: item control page