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Plant extracellular ATP signalling by plasma membrana NADPH oxidase and Ca2+ channels


Demidchik, V and Shang, Z and Shin, R and Thompson, E and Rubio, L and Laohavisit, A and Mortimer, JC and Chivasa, S and Slabas, AR and Glover, BJ and Schachtman, DP and Shabala, SN and Davies, JM, Plant extracellular ATP signalling by plasma membrana NADPH oxidase and Ca2+ channels, The Plant Journal, 58, (6) pp. 903-913. ISSN 0960-7412 (2009) [Refereed Article]

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Copyright Statement

Copyright 2009 Blackwell Publishing Ltd

DOI: doi:10.1111/j.1365-313X.2009.03830.x


Extracellular ATP regulates higher plant growth and adaptation. The signalling events may be unique to higher plants, as they lack animal purinoceptor homologues. Although it is known that plant cytosolic free Ca2+ can be elevated by extracellular ATP, the mechanism is unknown. Here, we have studied roots of Arabidopsis thaliana to determine the events that lead to the transcriptional stress response evoked by extracellular ATP. Root cell protoplasts were used to demonstrate that signalling to elevate cytosolic free Ca2+ is determined by ATP perception at the plasma membrane, and not at the cell wall. Imaging revealed that extracellular ATP causes the production of reactive oxygen species in intact roots, with the plasma membrane NADPH oxidase AtRBOHC being the major contributor. This resulted in the stimulation of plasma membrane Ca2+-permeable channels (determined using patch-clamp electrophysiology), which contribute to the elevation of cytosolic free Ca2+. Disruption of this pathway in the AtrbohC mutant impaired the extracellular ATP-induced increase in reactive oxygen species (ROS), the activation of Ca2+ channels, and the transcription of the MAP kinase3 gene that is known to be involved in stress responses. This study shows that higher plants, although bereft of purinoceptor homologues, could have evolved a distinct mechanism to transduce the ATP signal at the plasma membrane.

Item Details

Item Type:Refereed Article
Keywords:adenosine triphosphate, calcium, channel, MAP kinase, reactive oxygen species
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Shabala, SN (Professor Sergey Shabala)
ID Code:60241
Year Published:2009
Web of Science® Times Cited:153
Deposited By:Agricultural Science
Deposited On:2010-01-25
Last Modified:2012-10-11

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