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Strategies for the on-line preconcentration and separation of hypolipidaemic drugs using micellar electrokinetic chromatography


Dawod, M and Breadmore, MC and Guijt, RM and Haddad, PR, Strategies for the on-line preconcentration and separation of hypolipidaemic drugs using micellar electrokinetic chromatography, Journal of Chromatography A, 1217, (3) pp. 386-393. ISSN 0021-9673 (2010) [Refereed Article]

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DOI: doi:10.1016/j.chroma.2009.11.043


Three strategies were investigated for the simultaneous separation and on-line preconcentration of charged and neutral hypolipidaemic drugs in micellar electrokinetic chromatography (MEKC). A background electrolyte (BGE) consisting of 20 mM ammonium bicarbonate buffer (pH 8.50) and 50 mM sodium dodecyl sulfate (SDS) was used for the separation and on-line preconcentration of the drugs. The efficiencies of sweeping, analyte focusing by micelle collapse (AFMC), and simultaneous field-amplified sample stacking (FASS) and sweeping, were compared for the preconcentration of eight hypolipidaemic drugs in different conductivity sample matrices. When compared with a hydrodynamic injection (5 s at 50 mbar, 0.51% of capillary volume to detection window) of drug mixture prepared in the separation BGE, improvements of detection sensitivity of 60-, 83-, and 80-fold were obtained with sweeping, AFMC and simultaneous FASS and sweeping, respectively, giving limits of detection (LODs) of 50, 36, and 38 μg/L, respectively. The studied techniques showed suitability for focusing different types of analytes having different values of retention factor (k). This is the first report for the separation of different types of hypolipidaemic drugs by capillary electrophoresis (CE). The three methods were validated then applied for the analysis of target analytes in wastewater samples from Hobart city.

Item Details

Item Type:Refereed Article
Keywords:Hypolipidaemic drugs; Capillary electrophoresis; MEKC; Sweeping; Field-amplified sample stacking; Analyte focusing by micelle collapse; Pseudostationary phase
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Dawod, M (Mr Mohamed Dawod)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:59987
Year Published:2010
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:28
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-01-04
Last Modified:2011-08-12

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