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Purification and partial characterisation of striped trumpeter (Latris lineata) systemic immunoglobulin for the purpose of polyclonal anti-serum production

Citation

Covello, JM and Morrison, RN and Battaglene, SC and Nowak, BF, Purification and partial characterisation of striped trumpeter (Latris lineata) systemic immunoglobulin for the purpose of polyclonal anti-serum production, Aquaculture, 287, (1-2) pp. 11-17. ISSN 0044-8486 (2009) [Refereed Article]


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DOI: doi:10.1016/j.aquaculture.2008.10.013

Abstract

The striped trumpeter (Latris lineata) has been identified as a new species for diversification of the Tasmanian finfish culture industry. It is a deep water species with an unusually long, oceanic post-larval developmental stage and therefore, has not been easy to culture. Recent breakthroughs in the hatchery phase of culture have enabled the first sea cage grow-out trials. As the culture of striped trumpeter moves towards commercialscale grow-out, knowledge of the immune system as it relates to disease resistance and vaccination is becoming more important. This study began at the basic level of immunoglobulin (Ig) characterisation and then moved onto the creation of anti-serum, which was used as an immunological tool to investigate the onset of the antibody response in the striped trumpeter. Similar to many other teleost species, striped trumpeter Ig is composed of a light chain of Mr 283 kDa and a dominant heavy chain of Mr 867 kDa. As seen in many other species of teleosts, these heavy and light chains form a tetrameric molecule weighing approximately 926 kDa. Purified striped trumpeter Ig was used to create polyclonal anti-serum directed against the light chain. The anti-serum was then used to investigate the ontogeny of the antibody response. Using Western blot analysis, Ig could not be detected until larvae were 225 days post-hatch (dph). This is later in terms of days post-hatch than other fish examined and could affect future husbandry and vaccination practices for this species.

Item Details

Item Type:Refereed Article
Keywords:Immunoglobulin, Polyclonal antibody, Protein A, Latris lineata, Ontogeny
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Covello, JM (Ms Jennifer Covello)
Author:Morrison, RN (Dr Richard Morrison)
Author:Battaglene, SC (Associate Professor Stephen Battaglene)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:59950
Year Published:2009
Web of Science® Times Cited:5
Deposited By:TAFI - Marine Research Laboratory
Deposited On:2009-12-23
Last Modified:2010-04-22
Downloads:0

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