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Cloning and expression analysis of three striped trumpeter (Latris lineata) pro-inflammatory cytokines, TNF -α and IL-β, in response to infection by the ectoparasitic, Chondracanthus goldsmidi

Citation

Covello, JM and Bird, S and Morrison, RN and Battaglene, SC and Seacombes, CJ and Nowak, BF, Cloning and expression analysis of three striped trumpeter (Latris lineata) pro-inflammatory cytokines, TNF -a and IL-a, in response to infection by the ectoparasitic, Chondracanthus goldsmidi, Fish and Shellfish Immunology, 26, (5) pp. 773-786. ISSN 1050-4648 (2009) [Refereed Article]


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DOI: doi:10.1016/j.fsi.2009.03.012

Abstract

This study reports the cloning and sequencing of three striped trumpeter (Latris lineata Forster) proinflammatory cytokines, TNF-a, IL-1b and IL-8, as well as their differential expression in response to an infection by the ectoparasite Chondracanthus goldsmidi. The striped trumpeter TNF-a transcript consisted of 1093 bp, including a 759 bp ORF which translated into a 253 aa transmembrane peptide. The sequence contained a TACE cut site, that would produce a 167 aa soluble peptide containing the TNF ligand family signature. The IL-1b sequence consisted of 963 bp, including a 774 bp ORF which translated into a 258 aa protein. The protein lacked both a signal peptide and an ICE cleavage site, but did contain the IL-1 family signature. The sequence for the chemokine IL-8 contained 906 bp, with an ORF of 297 bp, which translated into a 99 aa protein. The protein lacked an ELR motif as is common with many teleost IL-8 sequences. The differential expression of the three cytokine genes in parasitized fish was investigated via quantitative real-time PCR. A significant up-regulation of all three pro-inflammatory cytokines was found in the gills, which were the site of parasite attachment. Examination of head kidney cells revealed a significant up-regulation of TNF-a, but not IL-1b or IL-8. Conversely, the spleen cells showed significant up-regulation of both IL-1b and IL-8, but not TNF-a. These findings allow for more detailed investigations of the striped trumpeter immune response.

Item Details

Item Type:Refereed Article
Keywords:Inflammatory response, TNF-a, IL-1b, IL-8, Ectoparasite
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Covello, JM (Ms Jennifer Covello)
Author:Morrison, RN (Dr Richard Morrison)
Author:Battaglene, SC (Associate Professor Stephen Battaglene)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:59949
Year Published:2009
Web of Science® Times Cited:53
Deposited By:TAFI - Marine Research Laboratory
Deposited On:2009-12-23
Last Modified:2015-02-02
Downloads:0

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