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Auxin Biosynthesis in Pea: Characterization of the Tryptamine Pathway
Citation
Quittenden, LJ and Davies, NW and Smith, Jason and Molesworth, PP and Tivendale, N and Ross, JJ, Auxin Biosynthesis in Pea: Characterization of the Tryptamine Pathway, Plant Physiology, 151, (3) pp. 1130-1138. ISSN 0032-0889 (2009) [Refereed Article]
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Copyright Statement
Copyright © 2009 by the American Society of Plant Biologists
Official URL: http://www.plantphysiol.org
DOI: doi:10.1104/pp.109.141507
Abstract
One pathway leading to the bioactive auxin, indole-3-acetic acid (IAA), is known as the tryptamine pathway, which is
suggested to proceed in the sequence: tryptophan (Trp), tryptamine, N-hydroxytryptamine, indole-3-acetaldoxime, indole-3-
acetaldehyde (IAAld), IAA. Recently, this pathway has been characterized by the YUCCA genes in Arabidopsis (Arabidopsis
thaliana) and their homologs in other species. YUCCA is thought to be responsible for the conversion of tryptamine to
N-hydroxytryptamine. Here we complement the genetic findings with a compound-based approach in pea (Pisum sativum),
detecting potential precursors by gas chromatography/tandem-mass spectrometry. In addition, we have synthesized
deuterated forms of many of the intermediates involved, and have used them to quantify the endogenous compounds, and
to investigate their metabolic fates. Trp, tryptamine, IAAld, indole-3-ethanol, and IAA were detected as endogenous
constituents, whereas indole-3-acetaldoxime and one of its products, indole-3-acetonitrile, were not detected. Metabolism
experiments indicated that the tryptamine pathway to IAA in pea roots proceeds in the sequence: Trp, tryptamine, IAAld, IAA,
with indole-3-ethanol as a side-branch product of IAAld. N-hydroxytryptamine was not detected, but we cannot exclude that it
is an intermediate between tryptamine and IAAld, nor can we rule out the possibility of a Trp-independent pathway operating
in pea roots.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant physiology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Grains and seeds not elsewhere classified |
| UTAS Author: | Quittenden, LJ (Dr Laura Quittenden) |
| UTAS Author: | Davies, NW (Associate Professor Noel Davies) |
| UTAS Author: | Smith, Jason (Professor Jason Smith) |
| UTAS Author: | Molesworth, PP (Dr Peter Molesworth) |
| UTAS Author: | Tivendale, N (Mr Nathan Tivendale) |
| UTAS Author: | Ross, JJ (Associate Professor John Ross) |
| ID Code: | 59936 |
| Year Published: | 2009 |
| Web of Science® Times Cited: | 67 |
| Deposited By: | Plant Science |
| Deposited On: | 2009-12-23 |
| Last Modified: | 2011-07-28 |
| Downloads: | 0 |
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