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Auxin regulation of gibberellin biosynthesis in the roots of pea (Pisum sativum)
journal contribution
posted on 2023-05-17, 01:02 authored by Weston, DE, James ReidJames Reid, John RossJohn RossAuxin promotes GA biosynthesis in the aboveground parts of plants. However, it has not been demonstrated previously that this interaction occurs in roots. To understand the interactions between auxin and GAs in these organs, we treated wild-type pea (Pisum sativum L.) roots with the inhibitors of auxin action, p-chlorophenoxyisobutyric acid (PCIB) and yokonolide B (YkB), and with the auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). These compounds generally downregulatedGAsynthesis genes and upregulatedGAdeactivation genes, and reduced the level of the bioactive GA1. These effects indicate that in pea roots, auxin at normal endogenous levels stimulates GA biosynthesis. We show also that supra-optimal levels of exogenous auxin reduce the endogenous level of bioactive GA in roots, although the effect appears too small to account for the strong growth-inhibitory effect of high auxin levels.
History
Publication title
Functional Plant Biology: An International Journal of Plant FunctionVolume
36Issue
4Pagination
362-369ISSN
1445-4408Department/School
School of Natural SciencesPublisher
C S I R O PublishingPlace of publication
150 Oxford St, Po Box 1139, Collingwood, Australia, Victoria, 3066Rights statement
Copyright © 2009 CSIRORepository Status
- Restricted