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Micelle to solvent stacking of organic cations in capillary zone electrophoresis with electrospray ionization mass spectrometry

Citation

Quirino, JP, Micelle to solvent stacking of organic cations in capillary zone electrophoresis with electrospray ionization mass spectrometry , The Journal of Chromatography A, 1216, (2) pp. 294-299. ISSN 0021-9673 (2009) [Refereed Article]


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DOI: doi:10.1016/j.chroma.2008.11.062

Abstract

The direction of the effective electrophoretic mobility of small organic cations in micellar electrokinetic chromatography using sodium dodecyl sulphate in a low-pH electrolyte can be reversed in the presence of organic solvent. This effective electrophoretic mobility change is presented here as a new dimension for on-line sample preconcentration of cations in capillary zone electrophoresis (CZE) using a background solution (BGS) modified by an organic solvent. The sample is prepared in a micellar solution without organic solvent. The focusing effect relies on the reversal in the effective electrophoretic mobility at the boundary zone between the micellar matrix and the BGS modified with organic solvent. This on-line sample preconcentration technique, called micelle to solvent stacking (MSS) afforded more than an order of magnitude improvement in concentration sensitivity compared to typical CZE-UV or CZE-electrospray ionization (ESI) MS analysis. The calculated limit of detection (S/N = 3) for pindolol and metoprolol analysed by MSS-CZE-ESI-MS was found to be 0.03 and 0.01 g/mL, respectively.

Item Details

Item Type:Refereed Article
Keywords:On-line sample preconcentration; Capillary zone electrophoresis; Electrospray ionization mass spectrometry; Adrenergic -blockers; Micelle to solvent stacking
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:59907
Year Published:2009
Web of Science® Times Cited:68
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-12-21
Last Modified:2014-10-17
Downloads:0

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