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Dry film microchips for miniaturised separations
Citation
Guijt, RM and Candish, E and Breadmore, MC, Dry film microchips for miniaturised separations, Electrophoresis, 30, (24) pp. 4219-4224. ISSN 0173-0835 (2009) [Refereed Article]
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Copyright Statement
The definitive published version is available online at: http://interscience.wiley.com
Official URL: http://interscience.wiley.com
DOI: doi:10.1002/elps.200900233
Abstract
In this work microfluidic devices were made from the dry film photoresist Ordyl SY330, characterised by optical and electron microscopy and used for electrophoretic separations. A simple and fast microfabrication process was developed for the fabrication of channels that are 50 m wide and 30 m in height, requiring only the use of an office laminator, a hot plate, an exposure source and mask and an electric drill to make four microdevices in less than 1 h. The optical properties of the photoresist were studied and the resist showed significant absorbance below 370 nm and 570-630 nm, and had an optical transmission of 80% between 400 and 550 nm. Fluorescence emission over the region of maximum transmission was low allowing these devices to be used for fluorescence detection at 488/512 nm. Electrophoretic separation of APTS and three derivatised sugars was performed in 20 mM phosphate buffer, pH 2.5 with efficiencies of the three sugars of 40 000 plates (2 100 000 plates/m) within 30 s at a field strength of 500 V/cm. The simple fabrication process also allowed microchannels to be easily filled with chromatography particles before sealing, avoiding the challenging task of slurry packing, and the potential of these devices for liquid chromatography was demonstrated by the extraction of fluorescein onto anion exchange particles.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | APTS • Carbohydrate • Dry film resist • Microchip • Microfabrication |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical chemistry |
| Research Field: | Separation science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the chemical sciences |
| UTAS Author: | Guijt, RM (Dr Rosanne Guijt) |
| UTAS Author: | Candish, E (Ms Esme Candish) |
| UTAS Author: | Breadmore, MC (Professor Michael Breadmore) |
| ID Code: | 59588 |
| Year Published: | 2009 |
| Web of Science® Times Cited: | 14 |
| Deposited By: | Austn Centre for Research in Separation Science |
| Deposited On: | 2009-12-14 |
| Last Modified: | 2015-01-21 |
| Downloads: | 0 |
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