eCite Digital Repository

Optimizing the use of shed feathers for genetic analysis

Citation

Hogan, FE and Cooke, R and Burridge, CP and Norman, JA, Optimizing the use of shed feathers for genetic analysis, Molecular Ecology Resources, 8, (2007) pp. 1-7. ISSN 1755-098X (2008) [Refereed Article]


Preview
PDF
Restricted - Request a copy
184Kb
  

Copyright Statement

The definitive published version is available online at: http://interscience.wiley.com

Official URL: http://interscience.wiley.com

DOI: doi:10.1111/j.1471-8286.2007.02044.x

Abstract

Shed feathers obtained by noninvasive genetic sampling (NGS) are a valuable source of DNA for genetic studies of birds. They can be collected across a large geographical range and facilitate research on species that would otherwise be extremely difficult to study. A limitation of this approach is uncertainty concerning the quality of the extracted DNA. Here we investigate the relationship between feather type, feather condition and DNA quality (amplification success) in order to provide a simple, cost-effective method for screening samples prior to genetic analysis. We obtained 637 shed feathers of the powerful owl (Ninox strenua) from across its range in southeastern Australia. The extracted DNA was amplified using polymerase chain reaction for a range of markers including mitochondrial DNA, ND3 and nuclear DNA, a simple sequence repeat (Nst02) and a portion of the CHD-1 gene (P2/P8). We found that feather condition significantly influenced the amplification success of all three loci, with feathers characterized as 'good' having greater success. Feather type was found to be of lower importance, with good quality feathers of all types consistently producing high success for all three loci. We also found that the successful amplification of multilocus genotypes was dependant on the condition of the starting material and was highly correlated with successful amplification of the sex-linked CHD-1 locus. Samples with low DNA quality have a higher probability of amplification failure and are more likely to produce incorrect genotypes; therefore, identifying samples with high DNA quality can save substantial time and cost associated with the genetic analysis of NGS. As a result, we propose a method for screening shed feathers in order to provide a subset of samples which will have a greater probability of containing high quality DNA suitable for the amplification of multilocus genotypes.

Item Details

Item Type:Refereed Article
Keywords:DNA quality, microsatellite genotyping, mitochondrial DNA, noninvasive sampling, sexing, shed feather
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Forest and Woodlands Flora, Fauna and Biodiversity
Author:Burridge, CP (Dr Christopher Burridge)
ID Code:59473
Year Published:2008
Web of Science® Times Cited:40
Deposited By:Zoology
Deposited On:2009-12-02
Last Modified:2010-06-08
Downloads:3 View Download Statistics

Repository Staff Only: item control page