In addition to increased glucose uptake, insulin action is associated with increased total and microvascular blood flow, and vasomotion in skeletal muscle. The aim of this study was to determine the effect of acute insulin resistance caused by the peripheral vasoconstrictor á-methylserotonin (áMT) on microvascular vasomotion in muscle. Heart rate (HR), mean arterial pressure (MAP), femoral blood flow (FBF), whole body glucose infusion (GIR) and hindleg glucose uptake (HGU) were determined during control and hyperinsulinaemic euglycaemic clamp conditions in anaesthetized rats receiving áMT infusion. Changes inmuscle microvascular perfusion were measured by laserDoppler flowmetry (LDF) and vasomotion was assessed by applying wavelet analysis to the LDF signal. Insulin increased GIR and HGU. Five frequency bands corresponding to cardiac, respiratory, myogenic, neurogenic and endothelial activities were detected in the LDF signal. Insulin infusion alone increased FBF (1.18±0.10 to 1.78±0.12 ml min–1, P<0.05), LDF signal strength (by 16% compared to baseline) and the relative amplitude of the myogenic component of vasomotion (0.89±0.09 to 1.18±0.06, P<0.05).When infused aloneáMTdecreased LDFsignal strength and themyogenic component of vasomotion by 23% and 27% respectively compared to baseline, but did not affect HGU or FBF. Infusion of áMT during the insulin clamp decreased the stimulatory effects of insulin on GIR, HGU, FBF and LDF signal and blocked the myogenic component of vasomotion. These data suggest that insulin action to recruit microvascular flow may in part involve action on the vascular smoothmuscle to increase vasomotion in skeletalmuscle to thereby enhance perfusion and glucose uptake. These processes are impaired with this model of áMT-induced acute insulin resistance.

Item Type:	Refereed Article
Research Division:	Biomedical and Clinical Sciences
Research Group:	Cardiovascular medicine and haematology
Research Field:	Cardiology (incl. cardiovascular diseases)
Objective Division:	Health
Objective Group:	Clinical health
Objective Field:	Clinical health not elsewhere classified
UTAS Author:	Newman, JMB (Dr John Newman)
UTAS Author:	Dwyer, RM (Dr Renee Ross)
UTAS Author:	St Pierre, P (Mr Philippe St Pierre)
UTAS Author:	Richards, SM (Dr Stephen Richards)
UTAS Author:	Clark, MG (Professor Michael Clark)
UTAS Author:	Rattigan, S (Professor Stephen Rattigan)
ID Code:	59123
Year Published:	2009
Web of Science® Times Cited:	56
Deposited By:	Menzies Institute for Medical Research
Deposited On:	2009-11-19
Last Modified:	2012-12-12
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