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Fast ion chromatography using short anion exchange columns


Tyrrell, EC and Shellie, RA and Hilder, EF and Pohl, CA and Haddad, PR, Fast ion chromatography using short anion exchange columns, The Journal of Chromatography A, 1216, (48) pp. 8512-8517. ISSN 0021-9673 (2009) [Refereed Article]

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DOI: doi:10.1016/j.chroma.2009.10.003


A fast ion chromatographic system is described which uses shorter column lengths and compares various eluent profiles in order to maximise the performance without sacrificing the chromatographic resolution. Both isocratic and gradient elution profiles were considered to find the most efficient mode of separation. The separation and determination of seven target anions (chloride, chlorate, nitrate, chromate, sulfate, thiocyanate and perchlorate) was achieved using a short (4 mm ID, 50 mm long) column packed with Dionex AS20 high-capacity anion exchange material. A hydroxide eluent was used at an initial concentration of 25 mM (at a flow-rate of 1.0 mL/min) and two performance maxima were found. The maximum efficiency occurred at a normalised gradient ramp rate of 5 mM/t0, resulting in a peak capacity of 16, while the fastest separation (<3 min) occurred at a normalised ramp rate of 30 mM/t0. The retention time, peak width and resolution using the different eluent profiles on varying column lengths is also compared. Further investigations in this study determined that the highest peak capacity separation under gradient conditions could be approximated using an isocratic separation. The advantage of using this novel approach to approximate the maximum efficiency separation removes the need for column re-equilibration that is required for gradient elution resulting in faster analyses and enhanced sample throughput, with benefits in particular for multidimensional chromatography.

Item Details

Item Type:Refereed Article
Keywords:Ion chromatography; Inorganic anions; Fast separation; Short columns; Gradient elution; Peak capacity
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Tyrrell, EC (Dr Eadaoin Tyrrell)
UTAS Author:Shellie, RA (Associate Professor Robert Shellie)
UTAS Author:Hilder, EF (Professor Emily Hilder)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:58929
Year Published:2009
Funding Support:Australian Research Council (DP0663781)
Web of Science® Times Cited:11
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-11-06
Last Modified:2010-04-08
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