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Fast ion chromatography using short anion exchange columns
Citation
Tyrrell, EC and Shellie, RA and Hilder, EF and Pohl, CA and Haddad, PR, Fast ion chromatography using short anion exchange columns, The Journal of Chromatography A, 1216, (48) pp. 8512-8517. ISSN 0021-9673 (2009) [Refereed Article]
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DOI: doi:10.1016/j.chroma.2009.10.003
Abstract
A fast ion chromatographic system is described which uses shorter column lengths and compares various eluent profiles in order to maximise the performance without sacrificing the chromatographic resolution. Both isocratic and gradient elution profiles were considered to find the most efficient mode of separation. The separation and determination of seven target anions (chloride, chlorate, nitrate, chromate, sulfate, thiocyanate and perchlorate) was achieved using a short (4 mm ID, 50 mm long) column packed with Dionex AS20 high-capacity anion exchange material. A hydroxide eluent was used at an initial concentration of 25 mM (at a flow-rate of 1.0 mL/min) and two performance maxima were found. The maximum efficiency occurred at a normalised gradient ramp rate of 5 mM/t0, resulting in a peak capacity of 16, while the fastest separation (<3 min) occurred at a normalised ramp rate of 30 mM/t0. The retention time, peak width and resolution using the different eluent profiles on varying column lengths is also compared. Further investigations in this study determined that the highest peak capacity separation under gradient conditions could be approximated using an isocratic separation. The advantage of using this novel approach to approximate the maximum efficiency separation removes the need for column re-equilibration that is required for gradient elution resulting in faster analyses and enhanced sample throughput, with benefits in particular for multidimensional chromatography.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Ion chromatography; Inorganic anions; Fast separation; Short columns; Gradient elution; Peak capacity |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| Author: | Tyrrell, EC (Dr Eadaoin Tyrrell) |
| Author: | Shellie, RA (Associate Professor Robert Shellie) |
| Author: | Hilder, EF (Professor Emily Hilder) |
| Author: | Haddad, PR (Professor Paul Haddad) |
| ID Code: | 58929 |
| Year Published: | 2009 |
| Funding Support: | Australian Research Council (DP0663781) |
| Web of Science® Times Cited: | 11 |
| Deposited By: | Austn Centre for Research in Separation Science |
| Deposited On: | 2009-11-06 |
| Last Modified: | 2010-04-08 |
| Downloads: | 2 View Download Statistics |
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