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Reversed Phase Liquid Chromatographic Method for the High-Throughput Analysis of Clopidogrel in Pharmaceutical Formulations Using a Monolithic Silica Column
journal contribution
posted on 2023-05-17, 00:15 authored by Aboul-Enein, HY, Hoenen, H, Ghanem, A, Koll, MA high-throughput, high performance liquid chromatographic method was developed and validated for the determination of clopidogrel in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed phase monolithic silica column Chromolith Performance 18e (100 mmtimes4.6 mm I.D.). The mobile phase consisted of acetonitrile:phosphate buffer (50:50 v/v, pH 3.0) at a flow rate of 4.0 mL/min. The photodiode array detector was set at 235 nm. The developed method showed a good linear relationship in the concentration range from 1.0 to 40.0 µg/mL with a correlation coefficient of 0.999. The limit of detection and limit of quantification were 0.97 µg/mL and 3.52 µg/mL, respectively.
History
Publication title
Journal of Liquid Chromatography and Related TechnologiesVolume
28Issue
9Pagination
1357-1365ISSN
1082-6076Department/School
School of Natural SciencesPublisher
Marcel Dekker IncPlace of publication
270 Madison Ave, New York, USA, Ny, 10016Rights statement
The definitive published version is available online at: http://www.tandf.co.uk/journalsRepository Status
- Restricted