eCite Digital Repository
Determination of Vardenafil in pharmaceutical formulation by HPLC using conventional C18 and monolithic silica columns
Citation
Aboul-Enein, HY and Ghanem, A and Hoenen, H, Determination of Vardenafil in pharmaceutical formulation by HPLC using conventional C18 and monolithic silica columns, Journal of Liquid Chromatography and Related Technologies, 28, (4) pp. 593-604. ISSN 1082-6076 (2005) [Refereed Article]
![]() | PDF Not available 204Kb |
Copyright Statement
The definitive published version is available online at: http://www.tandf.co.uk/journals
Official URL: http://www.tandf.co.uk/journals
DOI: doi:10.1081/JLC-200047217
Abstract
A simple HPLC analysis for the identification and quantification of vardenafil in a pharmaceutical tablet formulation was performed on a conventional C18 and Chromolith Performance RP-18e monolithic columns with acetonitrile-phosphate buffer mixtures as mobile phases. The effects of the proportion of organic solvent (from 20% to 90%), phosphate buffer pH (from 2 to 7.5) and flow rate (from 1 to 5 mL/min) were studied. The best chromatographic conditions were 20:80 (v/v) acetonitrile-10 mM phosphate buffer, pH 3.0, as mobile phase at 1 mL/min flow rate for the C18 column, whereas 30:70 (v/v) acetonitrile-10 mM phosphate buffer, pH 3.0, as mobile phase at 2 mL/min flow rate was best for the monolithic column. Methanol was found to be a suitable solvent for extraction of the active substance from tablets. For the C18 and monolithic column, the calibration plots were linear (R2 = 0.9996 and 0.9997, respectively) in the concentration range 10-1000 µg/mL. Limit of detection (LOD) and limit of quantification (LOQ) values were 0.10 and 0.31 µg/mL for the C18 and 0.11 mL and 0.32 µg/mL for the monolithic column. Intra-assay and inter-assay precision studies reflected a high level of reliability and reproducibility of the method. The proposed method is selective, precise (RSD = 0.45%), and accurate (recovery = 103-107%) in both columns used.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | liquid chromatography, monolithic column, pharmaceutical preparation, Vardenafil |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Ghanem, A (Dr Ashraf Ghanem) |
ID Code: | 58307 |
Year Published: | 2005 |
Web of Science® Times Cited: | 15 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2009-09-25 |
Last Modified: | 2012-11-19 |
Downloads: | 1 View Download Statistics |
Repository Staff Only: item control page