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Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of tiaprofenic acid in nonstandard HPLC organic solvents
journal contribution
posted on 2023-05-17, 00:13 authored by Ghanem, A, Aboul-Enein, MN, El-Azzouny, A, El-Behairy, MF, Al-Humaidi, E, Alaidan, AA, Amin, K, Al-Ahdal, MNThe first straightforward lipase-catalyzed enantioselective access to enantiomerically enriched tiaprofenic acid as a versatile method in chiral separation of racemates is demonstrated. The latter was directly monitored by enantioselective HPLC using a 3,5-dimethylphenylcarbamate derivative of cellulose-based chiral stationary phase namely Chiralpak IB (the immobilized version of Chiralcel OD). Non-standard HPLC organic solvents were used as diluent to dissolve the difficult to dissolve enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization. The existence of a non-standard HPLC organic solvent (e.g., methyl tert-butyl ether) in the mobile phase composition is mandatory to accomplish the simultaneous enantioselective HPLC baseline separation of both substrate and product.
History
Publication title
ChiralityVolume
20Issue
8Pagination
871-877ISSN
0899-0042Department/School
School of Natural SciencesPublisher
Wiley-LissPlace of publication
Div John Wiley & Sons Inc, 605 Third Ave, New YorkRights statement
The definitive published version is available online at: http://interscience.wiley.comRepository Status
- Restricted