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Electrokinetic and hydrodynamic injection: making the right choice for capillary electrophoresis


Breadmore, MC, Electrokinetic and hydrodynamic injection: making the right choice for capillary electrophoresis, Bioanalysis, 1, (5) pp. 889-894. ISSN 1757-6180 (2009) [Refereed Article]

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Copyright 2009 future science group

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DOI: doi:10.4155/BIO.09.73


CE is a powerful liquid-phase separation technique that is an attractive alternative to HPLC because of its small sample requirements, high resolving power and excellent mass detection limits. While there are many similarities between the two techniques, there are also many differences, some obvious, some subtle. One of the often overlooked differences is the way sample is injected. In HPLC, injection is a very minor component of the method and the choice is predominantly restricted to the choice of solvent and the injection volume. But in CE, it is vastly more complex, and development of an appropriate injection strategy should be given consideration during any method development. While the choice between hydrodynamic or electrokinetic injection may not initially be given any thought, selection of the right approach for the right application can lead to significant improvements in performance, particularly with regard to achieving the lowest detection limits possible. The question is how to decide the best way to inject for each application?

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:58296
Year Published:2009
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:22
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-09-25
Last Modified:2010-04-14
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