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Solvent versatility of immobilized amylase and cellulose-based chiral stationary phases in enantioselective LC separation and monitoring of bio-catalyzed resolutions of acidic drugs in non-standard organic solvents
Citation
Ghanem, A and Aboul-Enein, MN and El-Azzouny, A and El-Behairy, MF, Solvent versatility of immobilized amylase and cellulose-based chiral stationary phases in enantioselective LC separation and monitoring of bio-catalyzed resolutions of acidic drugs in non-standard organic solvents, Chromatographia, 70, (3-4) pp. 349-363. ISSN 0009-5893 (2009) [Refereed Article]
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The original publication is available at www.springerlink.com
Official URL: http://www.springerlink.com
DOI: doi:10.1365/s10337-009-1201-1
Abstract
The solvent versatility of Chiralpak IA (amylose tris(3,5-dimethylphenylcarbamate)), Chiralpak IB (cellulose tris(3,5-dimethylphenylcarbamate)) and Chiralpak IC (cellulose tris(3,5-dichlorophenylcarbamate)) immobilized onto silica gel, are investigated for the enantioselective separation of a set of acidic drugs in liquid chromatography. Non-standard LC organic solvents like dichloromethane, ethyl acetate, tetrahydrofuran, methyl-tert-butyl ether were used in mobile phase compositions and/or diluent agent for the analyte on all new columns. Furthermore, the enantioselective separations of the reported compounds were compared on both immobilized columns (Chiralpak IA and IB) and their conventionally coated versions (Chiralpak AD-H and Chiralcel OD-H, respectively), using a mixture of n-hexane/2-PrOH/TFA (80:20:0.1 v/v/v). The versatility of the immobilized Chiralpak IB in monitoring reactions performed in non-standard solvent was studied on a representative example consisting of the lipase-catalyzed enantioselective esterification of flurbiprofen with n-butanol in methyl-tert-butyl ether as organic solvent.
Item Details
Item Type: | Refereed Article |
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Keywords: | Column liquid chromatography - Enantioselective separation - Immobilized chiral stationary phase - Acidic drugs - Amylose tris(3,5-dimethylphenylcarbamate) and cellulose tris(3,5-dimethylphenylcarbamate) |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Ghanem, A (Dr Ashraf Ghanem) |
ID Code: | 58290 |
Year Published: | 2009 |
Web of Science® Times Cited: | 6 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2009-09-24 |
Last Modified: | 2010-04-08 |
Downloads: | 0 |
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