eCite Digital Repository

Solvent versatility of immobilized amylase and cellulose-based chiral stationary phases in enantioselective LC separation and monitoring of bio-catalyzed resolutions of acidic drugs in non-standard organic solvents

Citation

Ghanem, A and Aboul-Enein, MN and El-Azzouny, A and El-Behairy, MF, Solvent versatility of immobilized amylase and cellulose-based chiral stationary phases in enantioselective LC separation and monitoring of bio-catalyzed resolutions of acidic drugs in non-standard organic solvents, Chromatographia, 70, (3-4) pp. 349-363. ISSN 0009-5893 (2009) [Refereed Article]


Preview
PDF
Not available
508Kb
  

Copyright Statement

The original publication is available at www.springerlink.com

Official URL: http://www.springerlink.com

DOI: doi:10.1365/s10337-009-1201-1

Abstract

The solvent versatility of Chiralpak IA (amylose tris(3,5-dimethylphenylcarbamate)), Chiralpak IB (cellulose tris(3,5-dimethylphenylcarbamate)) and Chiralpak IC (cellulose tris(3,5-dichlorophenylcarbamate)) immobilized onto silica gel, are investigated for the enantioselective separation of a set of acidic drugs in liquid chromatography. Non-standard LC organic solvents like dichloromethane, ethyl acetate, tetrahydrofuran, methyl-tert-butyl ether were used in mobile phase compositions and/or diluent agent for the analyte on all new columns. Furthermore, the enantioselective separations of the reported compounds were compared on both immobilized columns (Chiralpak IA and IB) and their conventionally coated versions (Chiralpak AD-H and Chiralcel OD-H, respectively), using a mixture of n-hexane/2-PrOH/TFA (80:20:0.1 v/v/v). The versatility of the immobilized Chiralpak IB in monitoring reactions performed in non-standard solvent was studied on a representative example consisting of the lipase-catalyzed enantioselective esterification of flurbiprofen with n-butanol in methyl-tert-butyl ether as organic solvent.

Item Details

Item Type:Refereed Article
Keywords:Column liquid chromatography - Enantioselective separation - Immobilized chiral stationary phase - Acidic drugs - Amylose tris(3,5-dimethylphenylcarbamate) and cellulose tris(3,5-dimethylphenylcarbamate)
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Ghanem, A (Dr Ashraf Ghanem)
ID Code:58290
Year Published:2009
Web of Science® Times Cited:5
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-09-24
Last Modified:2010-04-08
Downloads:0

Repository Staff Only: item control page