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Two-dimensional ion chromatography using tandem ion-exchange columns with gradient-pulse column switching


Johns, CA and Shellie, RA and Pohl, CA and Haddad, PR, Two-dimensional ion chromatography using tandem ion-exchange columns with gradient-pulse column switching, The Journal of Chromatography A, 1216, (41) pp. 6931-6937. ISSN 0021-9673 (2009) [Refereed Article]

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DOI: doi:10.1016/j.chroma.2009.08.018


A two-dimensional ion chromatography (2D-IC) approach has been developed which provides greater resolution of complex samples than is possible currently using a single column. Two columns containing different stationary phases are connected via a tee-piece, which enables an additional eluent flow and independent control of eluent concentration on each column. The resultant mixed eluent flow at the tee-piece can be varied to produce a different eluent concentration on the second column. This allows analytes strongly retained on the first column to be separated rapidly on the second column, whilst maintaining a highly efficient, well resolved separation of analytes retained weakly on the first column. A group of 18 inorganic anions has been separated to demonstrate the utility of this approach and the proposed 2D-IC method provided separation of this mixture with resolution of all analytes greater than 1.3. Careful optimisation of the eluent profiles on both columns resulted in run times of less than 28 min, including re-equilibration. Separations were performed using isocratic or gradient elution on the first column, with an isocratic separation being used on the second column. Switching of the analytes onto the second column was performed using a gradient pulse of concentrated eluent to quickly elute strongly retained analytes from the first column onto the second column. The separations were highly repeatable (RSD of 0.01–0.12% for retention times and 0.08–2.9% for peak areas) and efficient (typically 8000–260,000 plates). Detection limits were 3–80 ppb.

Item Details

Item Type:Refereed Article
Keywords:Ion chromatography; Anions; Multidimensional chromatography
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Johns, CA (Dr Cameron Johns)
UTAS Author:Shellie, RA (Professor Robert Shellie)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:58289
Year Published:2009
Funding Support:Australian Research Council (DP0663781)
Web of Science® Times Cited:17
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-09-24
Last Modified:2014-11-21

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