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Protein systhesis in a solitary benthic cephalopod, the Southern dumpling squid ( Euprymna tasmanica)
Citation
Carter, CG and Lynch, KA and Moltschaniwskyj, NA, Protein systhesis in a solitary benthic cephalopod, the Southern dumpling squid ( Euprymna tasmanica), Comparative Biochemistry and Physiology. Part A: Molecular and Integrative Physiology, 153, (2) pp. 185-190. ISSN 1095-6433 (2009) [Refereed Article]
DOI: doi:10.1016/j.cbpa.2009.02.015
Abstract
Rates of protein synthesis were measured in the whole body and tissues of southern dumpling squid Euprymna
tasmanica to validate the use of a flooding-dose of 3H phenylalanine for the measurement of protein
synthesis with different size squid and to make a preliminary investigation into the effects of feeding regime.
In smaller (2.8±0.5 g, mean±SE) and larger (14.8±2.2 g) squid whole body fractional rates of protein
synthesis were 9.45±1.21 and 1.49±0.29% d−1, respectively. Differences in total whole body protein
content meant there was no difference in absolute rates of whole body protein synthesis between the larger
and smaller squid. In larger squid, fractional rates of protein synthesis were significantly higher in the
digestive gland (9.24±1.63% d−1) than in the arm tissue (1.43±0.31% d−1), which were significantly higher
than in the anterior (0.56±0.13% d−1) and posterior (0.36±0.04% d−1) mantle. In smaller squid there were
no differences in protein synthesis between tissues and high individual variation, due to differences in
feeding, was a likely cause. Consequently, the effect of feeding regime on protein synthesis was compared
between two groups of individually held squid: daily-feeding and minimal-feeding squid. The daily-feeding
squid had significantly higher feed intake, gained mass and had a significantly higher growth rate than the
minimal-feeding squid which lost mass. Whole body protein synthesis was significantly higher in the dailyfeeding
squid as was the protein content of the digestive gland, anterior and posterior mantle. There were
few other differences in indices of protein metabolism. Individual squid showed differences in growth and
protein metabolism, and there were significant relationships between growth rate and both rates of protein
synthesis and protein degradation. Thus, higher individual growth was a consequence of increased protein
synthesis, decreased protein degradation and, therefore, increased efficiency of retaining synthesised protein.
© 2009 Elsevier Inc. All rights reserved
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Biological Sciences |
Research Group: | Zoology |
Research Field: | Zoology not elsewhere classified |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Carter, CG (Professor Chris Carter) |
UTAS Author: | Lynch, KA (Mrs Kerri Lynch) |
UTAS Author: | Moltschaniwskyj, NA (Associate Professor Natalie Moltschaniwskyj) |
ID Code: | 58180 |
Year Published: | 2009 |
Web of Science® Times Cited: | 13 |
Deposited By: | NC Marine Conservation and Resource Sustainability |
Deposited On: | 2009-09-11 |
Last Modified: | 2012-03-05 |
Downloads: | 0 |
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