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Direct Enantioselective HPLC Monitoring of Lipase-Catalyzed Kinetic Resolution of 2-Phenoxy Propionic Acid in Non-Standard Organic Solvents
journal contribution
posted on 2023-05-17, 00:01 authored by Ghanem, A, El-Behairy, F, Al-Ahdal, MIn this work, a direct enantioselective HPLC monitoring system is developed. The system consists of an HPLC equipped with an immobilized cellulose-based chiral stationary phase Chiralpak IB, UV and a polarimetric detector in series. The developed system is used in the direct monitoring of the enantioselectivity of lipase-catalyzed enantioselective esterification of 2-phenoxy propionic acid of which the (R)-enantiomer is a precursor of the (R)-2-(p-hydroxyphenoxy)propionic acid known as an important intermediate in synthesizing aryloxyphenoxypropionic acid based agrochemicals. The versatility of the biocatalyzed reaction in chiral separation to access to both enantiomers of racemic 2-phenoxy propionic acid is demonstrated. The solvent versatility of the immobilized chiral stationary phase used in the direct monitoring of such reaction in non-standard HPLC solvent is shown.
History
Publication title
ChromatographiaVolume
65Issue
11-12Pagination
681-686ISSN
0009-5893Department/School
School of Natural SciencesPublisher
SpringerPlace of publication
GermanyRights statement
The original publication is available at www.springerlink.comRepository Status
- Restricted