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In this work, a direct enantioselective HPLC monitoring system is developed. The system consists of an HPLC equipped with an immobilized cellulose-based chiral stationary phase Chiralpak IB, UV and a polarimetric detector in series. The developed system is used in the direct monitoring of the enantioselectivity of lipase-catalyzed enantioselective esterification of 2-phenoxy propionic acid of which the (R)-enantiomer is a precursor of the (R)-2-(p-hydroxyphenoxy)propionic acid known as an important intermediate in synthesizing aryloxyphenoxypropionic acid based agrochemicals. The versatility of the biocatalyzed reaction in chiral separation to access to both enantiomers of racemic 2-phenoxy propionic acid is demonstrated. The solvent versatility of the immobilized chiral stationary phase used in the direct monitoring of such reaction in non-standard HPLC solvent is shown.

Item Type:	Refereed Article
Keywords:	Column liquid chromatography - Enantioselective separation - Kinetic resolution - Phenoxy propionic acid - Lipases
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
Author:	Ghanem, A (Dr Ashraf Ghanem)
ID Code:	57767
Year Published:	2007
Web of Science® Times Cited:	4
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2009-08-12
Last Modified:	2009-08-13
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